Concomitant staining of mast and parietal cells in human gastric mucosa

Biotech Histochem. 1996 Sep;71(5):234-6. doi: 10.3109/10520299609117166.

Abstract

A simple technique for concomitant staining of mast and parietal cells in the same section is described. Mast cells were stained by alcian blue or astra blue in methanol-formalin-acetic acid fixed biopsies of gastric mucosa. Parietal cells were visualized by Dolichos biflorus lectin binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcian Blue / chemistry
  • Gastric Mucosa / cytology*
  • Humans
  • Indoles / chemistry
  • Lectins / chemistry
  • Mast Cells / cytology*
  • Parietal Cells, Gastric / cytology*
  • Plant Lectins*
  • Staining and Labeling / methods*

Substances

  • Indoles
  • Lectins
  • Plant Lectins
  • dolichos biflorus agglutinin
  • Astra Blue
  • Alcian Blue