This paper describes the application of computer-aided three-dimensional reconstruction to measurements of the length of the organ of Corti (mean +/- SD, 35.58 +/- 1.41 mm), scala tympani outer wall (40.81 +/- 1.97 mm), scala tympani inner wall (18.29 +/- 1.47 mm), and Rosenthal's canal center (15.98 +/- 1.33 mm) in eight adult male cochleas. The Rosenthal's canal center ranged between 1 3/4 and 2 turns, did not appear to be linearly related to the organ of Corti, and lay near the basal end of the latter. The length of the organ of Corti measured with three-dimensional reconstruction differed by 7.6% +/- 3.2% ("cutting angle difference") from that derived from traditional two-dimensional reconstruction on the plane perpendicular to the plane of section, and by 2.6% +/- 1.7% ("geometric difference") from that derived from two-dimensional reconstruction on the axial plane at right angles to the modiolar axis.