Angiogenesis is key to both the development and regeneration of the dentin-pulp complex.
Objective: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events.
Methods: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes.
Results: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity.
Conclusion: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration.
Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.