Abstract
Phytoecdysteroids, structurally similar to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. In skeletal muscle cells, phytoecdysteroids increase protein synthesis. In this study we show that in a mouse skeletal muscle cell line, C(2)C(12), 20-hydroxyecdysone (20HE), a common phytoecdysteroid in both insects and plants, elicited a rapid elevation in intracellular calcium, followed by sustained Akt activation and increased protein synthesis. The effect was inhibited by a G-protein coupled receptor (GPCR) inhibitor, a phospholipase C (PLC) inhibitor, and a phosphoinositide kinase-3 (PI3K) inhibitor.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Biological Transport / drug effects
-
Blotting, Western
-
Boron Compounds / pharmacology
-
Calcium / metabolism*
-
Cell Line
-
Chelating Agents / pharmacology
-
Ecdysteroids / pharmacology
-
Ecdysterone / pharmacology
-
Egtazic Acid / analogs & derivatives
-
Egtazic Acid / pharmacology
-
Estrenes / pharmacology
-
Mice
-
Muscle, Skeletal / cytology*
-
Muscle, Skeletal / drug effects
-
Muscle, Skeletal / metabolism*
-
Phosphatidylinositol 3-Kinases / metabolism
-
Phosphoinositide-3 Kinase Inhibitors
-
Phosphorylation / drug effects
-
Proto-Oncogene Proteins c-akt / metabolism*
-
Pyrrolidinones / pharmacology
-
Type C Phospholipases / antagonists & inhibitors
-
Type C Phospholipases / metabolism
Substances
-
Boron Compounds
-
Chelating Agents
-
Ecdysteroids
-
Estrenes
-
Phosphoinositide-3 Kinase Inhibitors
-
Pyrrolidinones
-
1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
-
Egtazic Acid
-
Ecdysterone
-
2-aminoethoxydiphenyl borate
-
Proto-Oncogene Proteins c-akt
-
Type C Phospholipases
-
Calcium