Abstract
This study investigated the potential use of platelet-rich plasma (PRP) in conjunction with mRNA expression of bone matrix proteins using bioassay and RT-PCR comparing bovine bone morphogenetic proteins (BMP), recombinant human BMP-4 (rhBMP-4) during rat bone marrow stromal cell (Mesenchymal Stem Cell) differentiation at 14 days. The results showed that all three growth factors were associated with significantly elevated alkaline phosphatase activity. PRP and bovine BMP resulted in increased protein content. The mRNA of type I collagen was expressed with all three growth factors and remained consistently elevated. Osteopontin was observed with PRP from days 1 to 7; bone sialoprotein expression was detected on days 1 and 3. PRP, bovine BMP and rhBMP-4 enhanced the steady-state expression of PDGF-A as time-dependent to day 14 and in PRP was the strongest. PTHr was expressed at days 1 and 5. Vascular endothelial growth factor expression was the most highly expressed after day 3. These findings suggest that PRP increases mRNA expression of bone matrix protein, enchances osteogenesis and angiogenesis in vitro.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism
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Animals
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Bone Marrow Cells / cytology*
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Bone Marrow Cells / metabolism
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Bone Matrix / metabolism*
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Bone Morphogenetic Protein 4 / metabolism
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Bone Morphogenetic Protein 4 / pharmacology*
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Bone Morphogenetic Proteins / metabolism
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Bone Morphogenetic Proteins / pharmacology*
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Cattle
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Cell Differentiation*
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Cells, Cultured
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Collagen Type I / genetics
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Collagen Type I / metabolism
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Humans
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Integrin-Binding Sialoprotein
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Neovascularization, Physiologic / drug effects
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Osteogenesis / drug effects
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Osteopontin / genetics
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Osteopontin / metabolism
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Platelet-Derived Growth Factor / genetics
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Platelet-Derived Growth Factor / metabolism
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Platelet-Rich Plasma / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Sialoglycoproteins / genetics
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Sialoglycoproteins / metabolism
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Stromal Cells / cytology
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Stromal Cells / metabolism
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism
Substances
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BMP4 protein, human
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Bone Morphogenetic Protein 4
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Bone Morphogenetic Proteins
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Collagen Type I
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IBSP protein, human
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Ibsp protein, rat
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Integrin-Binding Sialoprotein
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Platelet-Derived Growth Factor
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RNA, Messenger
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Sialoglycoproteins
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Vascular Endothelial Growth Factor A
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platelet-derived growth factor A
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Osteopontin
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Alkaline Phosphatase