Abstract
The objective of this study was to analyze the proliferative response of BALB/c mice lymphocytes after in vitro irradiation (0.05 to 6 Gy). The capability of irradiated lymphocytes for proliferating without any stimulation and after activation with specific T and B cell mitogens has been evaluated. The results show that ionizing radiation significantly inhibits spontaneous cellular proliferation and that induced by mitogens and that variations in the degree of inhibition are found depending on the inducing proliferation mitogens and the dosage applied. The conclusion drawn is that different lymphocyte populations have different radiosensitivities, being B cells more sensitive to ionizing irradiation than T cells. Besides, the effects of gamma-irradiation vary according to the different subpopulations of T cells or, alternatively, to different T-dependent activation mechanisms.
MeSH terms
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Animals
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / drug effects
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / radiation effects
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Cell Proliferation / drug effects*
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Cell Proliferation / radiation effects*
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Cells, Cultured
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Concanavalin A / administration & dosage
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Dose-Response Relationship, Immunologic
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Dose-Response Relationship, Radiation
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Female
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Gamma Rays*
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Lipopolysaccharides / administration & dosage
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / radiation effects
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Male
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Mice
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Mice, Inbred BALB C
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Mitogens / administration & dosage*
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Mitogens / immunology
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Phytohemagglutinins / administration & dosage
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Pokeweed Mitogens / administration & dosage
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Radiation Tolerance / immunology
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T-Lymphocyte Subsets / cytology
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T-Lymphocyte Subsets / drug effects
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / radiation effects
Substances
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Lipopolysaccharides
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Mitogens
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Phytohemagglutinins
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Pokeweed Mitogens
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Concanavalin A