Abstract
Dermal papilla cells of the hair follicle can be maintained in an active, hair-inducing state in vitro when cocultured with cells secreting Wnt3a. By inducing cultured dermal papilla cells to secrete Wnt themselves, we demonstrate that this activity is a direct effect of Wnt signaling to dermal papilla cells. We further demonstrate that the effects of Wnt3a are exerted through activation of the beta-catenin signal transduction pathway and do not require alternative Wnt transduction cascades. Once dermal papilla cells have lost hair-inducing properties in vitro, neither treatment with Wnt nor expression of a truncated and activating form of beta-catenin is sufficient to restore these properties to the cultured cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Cytoskeletal Proteins / metabolism*
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Female
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Green Fluorescent Proteins
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Hair Follicle / cytology*
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Hair Follicle / metabolism*
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Indicators and Reagents / metabolism
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Luminescent Proteins / genetics
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Male
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Mice
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Mice, Transgenic
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Pregnancy
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Proteins / metabolism*
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Retroviridae / genetics
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Signal Transduction / physiology*
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Trans-Activators / metabolism*
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Wnt Proteins
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Wnt3 Protein
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Wnt3A Protein
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beta Catenin
Substances
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CTNNB1 protein, mouse
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Cytoskeletal Proteins
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Indicators and Reagents
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Luminescent Proteins
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Proteins
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Trans-Activators
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Wnt Proteins
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Wnt3 Protein
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Wnt3A Protein
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Wnt3a protein, mouse
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beta Catenin
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Green Fluorescent Proteins