[Experimental study on inhibitory effect of rutin against platelet activation induced by platelet activating factor in rabbits]

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2002 Apr;22(4):283-5.
[Article in Chinese]

Abstract

Objective: To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration.

Methods: The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay.

Results: Rutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently.

Conclusion: Rutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport, Active
  • Blood Platelets / metabolism
  • Calcium / metabolism
  • Male
  • Platelet Activating Factor / antagonists & inhibitors*
  • Platelet Activation / drug effects
  • Platelet Aggregation Inhibitors / pharmacology*
  • Rabbits
  • Rutin / pharmacology*
  • Serotonin / metabolism

Substances

  • Platelet Activating Factor
  • Platelet Aggregation Inhibitors
  • Serotonin
  • Rutin
  • Calcium