Abstract
Dendroside A (1) and dendronobilosides A and B (2 and 3), three new sesquiterpene glycosides, have been isolated from the stems of Dendrobium nobile, a plant used in Chinese traditional medicine. Their structures and stereochemistry were determined as 10beta,12,14-trihydroxyalloaromadendrane 14-O-beta-D-glucopyranoside (1), 10,12-dihydroxypicrotoxane 10,12-di-O-beta-D-glucopyranoside (2), and 6alpha,10,12-trihydroxypicrotoxane 10-O-beta-D-glucopyranoside (3), respectively, on the basis of spectroscopic and chemical methods. Quantum chemistry calculations were used in support of the structural determination of 1. Compounds 1 and 2 were found to stimulate the proliferation of murine T and B lymphocytes in vitro, while compound 3 showed inhibitory activity in this same assay.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adjuvants, Immunologic / chemistry
-
Adjuvants, Immunologic / isolation & purification*
-
Adjuvants, Immunologic / pharmacology
-
Animals
-
B-Lymphocytes / drug effects
-
B-Lymphocytes / immunology
-
B-Lymphocytes / metabolism
-
Chromatography, Thin Layer
-
Concanavalin A / pharmacology
-
Drugs, Chinese Herbal / chemistry
-
Drugs, Chinese Herbal / isolation & purification
-
Drugs, Chinese Herbal / pharmacology
-
Glucosides / chemistry
-
Glucosides / isolation & purification*
-
Glucosides / pharmacology
-
Glycosides / chemistry
-
Glycosides / isolation & purification*
-
Glycosides / pharmacology
-
In Vitro Techniques
-
Lipopolysaccharides / pharmacology
-
Lymphokines / chemistry
-
Lymphokines / isolation & purification*
-
Lymphokines / pharmacology
-
Magnetic Resonance Spectroscopy
-
Medicine, Chinese Traditional
-
Mice
-
Molecular Structure
-
Plant Lectins
-
Plant Stems / chemistry
-
Plants, Medicinal / chemistry*
-
Sesquiterpenes / chemistry
-
Sesquiterpenes / isolation & purification*
-
Sesquiterpenes / pharmacology
-
Spectrophotometry, Infrared
-
Spleen / cytology
-
Spleen / drug effects
-
Spleen / immunology
-
Spleen / metabolism
-
T-Lymphocytes / drug effects
-
T-Lymphocytes / immunology
-
T-Lymphocytes / metabolism
Substances
-
Adjuvants, Immunologic
-
Drugs, Chinese Herbal
-
Glucosides
-
Glycosides
-
Lipopolysaccharides
-
Lymphokines
-
Plant Lectins
-
Sesquiterpenes
-
dendronobiloside A
-
dendronobiloside B
-
dendroside A
-
lymphocyte proliferation potentiating factors
-
Concanavalin A