Protective effect of taurine on diabetic rat endothelial dysfunction

Natsuko Ikubo; Motoaki Saito; Panagiota Tsounapi; Fotios Dimitriadis; Fumiya Ohmasa; Seiya Inoue; Shogo Shimizu; Yukako Kinoshita; Keisuke Satoh

doi:10.2220/biomedres.32.187

Full Papers

Protective effect of taurine on diabetic rat endothelial dysfunction

Natsuko Ikubo, Motoaki Saito, Panagiota Tsounapi, Fotios Dimitriadis, Fumiya Ohmasa, Seiya Inoue, Shogo Shimizu, Yukako Kinoshita, Keisuke Satoh

Author information

Natsuko Ikubo
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Motoaki Saito
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Panagiota Tsounapi
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Fotios Dimitriadis
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Fumiya Ohmasa
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Seiya Inoue
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Shogo Shimizu
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Yukako Kinoshita
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan
Keisuke Satoh
Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Tottori University School of Medicine, 86 Nishi-machi, Yonago, 683-8503, Japan

JOURNAL FREE ACCESS

2011 Volume 32 Issue 3 Pages 187-193

DOI https://doi.org/10.2220/biomedres.32.187

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Abstract

As increasing evidence suggest that oxidative stress plays an important role in the developing angiopathy in diabetes, we studied the effects of taurine, a free radical scavenger, on diabetesinduced angiopathy in the rat aorta. Six-week-old male Wistar rats were randomly divided into three groups; control group (Cont), diabetes group (DM) and diabetes group treated with taurine for four weeks, 500 mg/kg/day, intraperitoneally (i.p.) (DM+T). Diabetes was induced by streptozotocin (50 mg/kg i.p.). Four weeks after the induction of diabetes, serum glucose and malondialdehyde concentrations were measured. Additionally, organ bath studies and real-time PCR on muscarinic M₃ receptor and eNOS were performed. Although taurine treatment failed to decrease serum glucose levels, the increased serum malondialdehyde levels in diabetic rats were significantly decreased after taurine treatment. Norepinephrine-induced hyper-contractility as well as acetylcholine-induced, endothelium-dependent hypo-relaxation in diabetes were significantly prevented after taurine treatment. The differences in the expressions of muscarinic M₃ receptor mRNAs were statistically non-significant between groups. Moreover, diabetes-induced up-regulation of eNOS mRNAs was slightly prevented after taurine treatment. These data suggest that taurine acts beneficially against the diabetes-induced vascular dysfunction. Its potential action as a radical scavenger ameliorates the vascular disorders in diabetes.

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