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Prognostic Relevance of Tensin4 Expression in Human Gastric Cancer

  • Laboratory Research
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Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

The Tensin4 gene is involved in various biological events by mediating signal transduction. This study was designed to clarify its clinical significance in gastric cancer.

Methods

A total of 114 gastric cancer patients were enrolled in this study, and we obtained paired samples from tumor tissue and matched normal mucosa. Total RNA was extracted, and TNS4 mRNA expression was quantified using real-time reverse-transcription polymerase chain reaction. TNS4 protein expression was investigated by immunohistochemistry. Additionally, the relationship between TNS4 expression and clinicopathologic factors was statistically analyzed.

Results

TNS4 mRNA expression was significantly higher in tumor tissue than in normal mucosa (p < 0.0001). Additional immunohistochemical analysis demonstrated that TNS4 protein was predominantly expressed in gastric cancer. Tumors with higher TNS4 mRNA expression showed histologically poorer grade (p < 0.02), deeper invasion into the serosa (p < 0.01), more positive lymph node metastasis (p < 0.02) or peritoneal dissemination (p < 0.05). Patients with high TNS4 mRNA expression tumor were more likely to have cancer-related death (p < 0.05). A Kaplan–Meier curve also demonstrated that patients with high TNS4 expression tumor had significantly poorer prognosis (p < 0.01) than those with low TNS4 expression tumor. In particular, the 2-year overall survival rates were 48.2% and 83.1%, respectively, a very significant difference. Multivariate analysis revealed that high TNS4 mRNA expression was an independent prognostic predictor (relative risk 1.41, 95% confidence interval 1.03–1.96, p = 0.03).

Conclusion

TNS4 was frequently overexpressed in gastric cancer, and tumors with high TNS4 mRNA expression showed biologically aggressive behavior. High TNS4 mRNA expression may be a novel prognostic predictor for those patients.

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Acknowledgements

We thank T. Shimooka, K. Ogata, M. Oda, N. Kasagi, and Y. Nakagawa for their excellent technical assistance. This work was supported in part by the following grants and foundations: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; and The Ministry of Education, Culture, Sports, Science and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Area, grant number 18015039.

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Authors and Affiliations

  1. Department of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, Beppu, 874-0838, Japan

    Katsuya Sakashita MD, Koshi Mimori MD, PhD, Fumiaki Tanaka MD, PhD, Yukio Kamohara MD, PhD, Hiroshi Inoue MD, PhD & Masaki Mori MD, PhD, FACS

  2. Department of Surgical Oncology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka, 545-8585, Japan

    Katsuya Sakashita MD, Tetsuji Sawada MD, PhD & Kosei Hirakawa MD, PhD

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  1. Katsuya Sakashita MD
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  2. Koshi Mimori MD, PhD
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  3. Fumiaki Tanaka MD, PhD
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  4. Yukio Kamohara MD, PhD
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  5. Hiroshi Inoue MD, PhD
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  6. Tetsuji Sawada MD, PhD
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  7. Kosei Hirakawa MD, PhD
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  8. Masaki Mori MD, PhD, FACS
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Correspondence to Koshi Mimori MD, PhD.

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Sakashita, K., Mimori, K., Tanaka, F. et al. Prognostic Relevance of Tensin4 Expression in Human Gastric Cancer. Ann Surg Oncol 15, 2606–2613 (2008). https://doi.org/10.1245/s10434-008-9989-8

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  • DOI: https://doi.org/10.1245/s10434-008-9989-8

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