Original CommunicationsInsulin and glucocorticoid-dependent suppression of the IGF-I system in diabetic wounds*,**
Section snippets
Animals
Adult Wistar male rats (Kuwait University breeding colony) weighing 175 to 220 g at the beginning of the experiment were used. The rats were housed in groups of 5 in plastic cages in artificial lighting with a fixed 12-hour light-dark cycle. The ambient temperature was kept at 22°C, and the rats had free access to standard laboratory food and tap water. The study protocol was approved by the institutional animal investigation committee.
Diabetes induction and insulin treatment
Diabetes was induced in overnight-fasted rats by an
Biochemical and physical parameters
Five to 7 days after STZ injection, animals taken from various experimental groups—including vehicle-diabetic, insulin-diabetic, and RU-486-diabetic—were initially matched with regard to plasma levels of glucose (480 ± 35/100 mL ), insulin (0.90 ± 0.2 μU/mL), and hydroxybutyrate (1.35 ± 0.4 mmol/L). These indexes are commonly used as indicators of the severity of diabetes. Physical and biochemical evaluation of animals in various experimental groups revealed that the gain in body weight of the
Discussion
IGF-I alone or in connection with other growth-promoting polypeptides orchestrates different biochemical events that culminate in the restoration of functional integrity of wounded skin. Wound healing potential is diminished as a function of diabetes. Identification of the biochemical abnormalities responsible for the development of this phenomenon has remained a challenge for decades, and its is still not known whether nonhealing, cutaneous, diabetic wounds can be reversed in a clinical
Acknowledgements
We thank Ms Sabah Wahid, Dr Saju Abraham, and Dr Thameem Farook for their technical assistance and Prof C. W. T. Pilcher for his helpful comments and suggestions.
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Supported by Kuwait University Grant MR033.
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Reprint requests: Prof Milad S. Bitar, Department of Pharmacology, Faculty of Medicine, PO Box 24923, 13110 Safat, Kuwait.