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Biomed-2

Improved reliability of lymphoma diagnostics via PCR-based clonality testing: — Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

Leukemia volume 21, pages 201–206 (2007)Cite this article

Abstract

The diagnosis of malignant lymphoma is a recognized difficult area in histopathology. Therefore, detection of clonality in a suspected lymphoproliferation is a valuable diagnostic criterion. We have developed primer sets for the detection of rearrangements in the B- and T-cell receptor genes as reliable tools for clonality assessment in lymphoproliferations suspected for lymphoma. In this issue of Leukemia, the participants of the BIOMED-2 Concerted Action CT98-3936 report on the validation of the newly developed clonality assays in various disease entities. Clonality was detected in 99% of all B-cell malignancies and in 94% of all T-cell malignancies, whereas the great majority of reactive lesions showed polyclonality. The combined BIOMED-2 results are summarized in a guideline, which can now be implemented in routine lymphoma diagnostics. The use of this standardized approach in patients with a suspect lymphoproliferation will result in improved diagnosis of malignant lymphoma.

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Acknowledgements

The successful completion of the EU-supported BIOMED-2 Concerted Action BMH4-CT98-3936 was based on efficient and open collaboration of 47 institutes with the following active participants:Netherlands: JJM van Dongen and AW Langerak, Department of Immunology, Erasmus MC, Rotterdam; PhM Kluin and E Schuuring, Department of Pathology and Laboratory Medicine, Academic Hospital Groningen, Groningen; JHJM van Krieken and PJTA Groenen, Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen; AH Mulder, Department of Pathology, Rijnstate Hospital, Arnhem; ST Pals and M Spaargaren, Department of Pathology, Academic Medical Center, Amsterdam; Belgium: E Moreau and E Boone, H Hartziekenhuis, Roeselare; Spain: JF San Miguel, R García Sanz, M Gonzalez Diaz and D Gonzalez, Department of Hematology, Universidad de Salamanca, Salamanca; T Flores Corral, Anatomia Patologica, Universidad de Salamanca, Salamanca; MA Piris, R Villuendas, B Martinez Delgado, and JF Garcia, Programa de Patologia Molecular, Centro Nacional de Investigaciones Oncológicas, Madrid; Portugal: A Parreira, J Diamond, P Gameiro, and R Fragoso, Instituto Portoguês de Oncologia, Lisbon; JM Cabeçadas, Department of Pathology, Instituto Português de Oncologia, Lisbon; C Sambade, Department of Pathology, Institute of Mol Pathology and Immunology of the University of Porto, Porto; United Kingdom: JL Smith, L Lavender, and E Hodges, Molecular Pathology Unit, Southampton University Hospitals, Southampton; L Lavender, Molecular Genetics Diagnostic Laboratory, St George's Hospital, London; H White, National Genetics Reference Laboratory, Salisbury District Hospital, Wiltshire; L Foroni, Department of Haematology, Royal Free Hospital, London; TC Diss and P Isaacson, Department of Histopathology, UCL Medical School; BS Wilkins, Histopathology Department, Royal Victoria Infirmary, Newcastle upon Tyne; B Jasani and K Mills, University of Wales, Cardiff; GJ Morgan, Department of Hemato Oncology, Institute of Cancer Research, Sutton Surrey; PA Evans, and A Jack, Haematological Malignancy Diagnostic Service, General Infirmary, Leeds; D Pearson, Department of Pathology, Cambridge University, Cambridge; I Carter, Department of Molecular Diagnostics and Histopathology, Nottingham City Hospital NHS Trust, Nottingham; B Jennings, School of Medicine, University of East Anglia, Norwich; BJ Milner, Department of Medicine and Therapeutics, Aberdeen University, Aberdeen; M Vickers, Department of Haematology, Aberdeen Royal Infirmary, Aberdeen; Germany: M Kneba, C Pott, M Brüggemann, and J Droese, II Medizinische Klinik der Universität Kiel, Kiel; H Herbst and C Kersting, Gerhard-Domagk Institut für Pathologie, Münster; M Hummel and H Stein, Institute of Pathology, Free University Berlin, Berlin; CR Bartram and T Flohr, Institute of Human Genetics, University of Heidelberg, Heidelberg; L Trümper and W Jung, Department of Internal Medicine, Georg August University of Göttingen, Göttingen; M Ott and P Starostik, Institute of Pathology, Würzburg University, Würzburg; R Parwaresch and M Tiemann, Institute for Hematopathology, University of Kiel, Kiel; ML Hansmann and S Oeschger, Department of Pathology, Johann Wolfgang Goethe University Hospital, Frankfurt; France: EA Macintyre, E Delabesse, K Beldjord, and V Asnafi, Laboratoire d’Hematologie, Hôpital Necker-Enfants Malades, Paris; C Bastard and S Laberge, Centre Henri Becquerel, Rouen; F Davi and F Charlotte, Hopital Pitié-Salpétrière, Paris; MH Delfau-Larue, Service d’Immunologie Biologique, Hopital Henri Mondor-CHU Creteil, Creteil; G Delsol and T Al Saati, Lab d’Anatomie Pathologique, Hopital Purpan, Toulouse; TJ Molina, Department of Pathology, Hotel-Dieu de Paris, Paris; G Salles, F Berger, and L Baseggio, Centre Hospitalier Lyon-Sud, Pierre-Benite; D Canioni, Service d’Anatomie Pathologique, Hôpital Necker-Enfants Malades, Paris; P Gaulard and C Copie, Department de Pathologie, Hopital Henri Mondor-CHU Creteil, Creteil.

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Authors and Affiliations

  1. Department of Pathology, UMC St Radboud, Nijmegen, The Netherlands

    J H J M van Krieken & P J T A Groenen

  2. Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands

    A W Langerak & J J M van Dongen

  3. Laboratoire d’Hematologie, Hôpital Necker-Enfants Malades, Paris, France

    E A Macintyre

  4. II Medical Department, University Hospital Schleswig-Holstein, Kiel, Germany

    M Kneba, M Brüggemann & Ch Pott

  5. Department of Immunology, Southampton General Hospital, Southampton, UK

    E Hodges

  6. Department of Hematology, Hospital Universitario de Salamanca, Salamanca, Spain

    R Garcia Sanz

  7. Department of Molecular Haematology, University of Leeds, Leeds, UK

    G J Morgan & P A S Evans

  8. Department of Haematology, Instituto Portugués de Oncologia, Lisbon, Portugal

    A Parreira

  9. Department of Pathology, Hotel-Dieu de Paris, Paris, France

    T J Molina

  10. Department of Pathology, Instituto Portugués de Oncologia, Lisbon, Portugal

    J Cabeçadas

  11. Department of Pathology, CHU Henri Mondor, Creteil, France

    P Gaulard

  12. Department of Pathology, University of Wales College of Medicine, Cardif, UK

    B Jasani

  13. Molecular Pathology Program, CNIO, Madrid, Spain

    J F Garcia

  14. Department of Pathology, Luitpoldkrankenhaus, Würzburg, Germany

    M Ott

  15. Department of Pathology, Johann Wolfgang Goethe University Hospital, Frankfurt, Germany

    M L Hannsmann

  16. Department of Pathology, Centre Hospitalier Lyon-Sud, Pierre-Benite, France

    F Berger

  17. Institut für Pathologie, Charité – Universitätsmedizin Berlin, Berlin, Germany

    M Hummel

  18. Department of Haematology, Hopital Pitié-Salpétière, Paris, France

    F Davi

  19. Wessex Immunology Service, Molecular Pathology Unit, Southampton University Hospitals, Southampton, UK

    F L Lavender & H White

  20. Department of Pathology, University Medical Center Groningen, Groningen, The Netherlands

    E Schuuring

  21. Department of Haematology, Centre Hospitalier Lyon-Sud, Lyon, France

    G Salles

  22. Department of Molecular Biology, Instituto Português de Oncologia, Lisbon, Portugal

    P Gameiro

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  1. J H J M van Krieken
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  2. A W Langerak
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  3. E A Macintyre
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  4. M Kneba
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  7. G J Morgan
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  8. A Parreira
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  9. T J Molina
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  10. J Cabeçadas
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  13. J F Garcia
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  14. M Ott
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  15. M L Hannsmann
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  16. F Berger
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  17. M Hummel
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  18. F Davi
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  19. M Brüggemann
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  20. F L Lavender
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  21. E Schuuring
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  22. P A S Evans
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  23. H White
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  24. G Salles
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  25. P J T A Groenen
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  26. P Gameiro
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  27. Ch Pott
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  28. J J M van Dongen
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Correspondence to J J M van Dongen.

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van Krieken, J., Langerak, A., Macintyre, E. et al. Improved reliability of lymphoma diagnostics via PCR-based clonality testing: — Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia 21, 201–206 (2007). https://doi.org/10.1038/sj.leu.2404467

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