Abstract
Following the transfer into fertilized mouse eggs of recombinant genes composed of the upstream region of the rat insulin II gene linked to sequences coding for the large-T antigen of simian virus 40, large-T antigen is detected exclusively in the β-Cells of the endocrine pancreas of transgenic mice. The α- and δ-cells normally found in the islets of Langerhans are rare and disordered. Well-vascularized β-cell tumours arise in mice harbouring and inheriting these hybrid oncogenes.
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References
Steiner, D. F. & Freinkel, N. (eds) Endocrine Pancreas. Handbook of Physiology Vol. 1 (Williams & Wilkins, Baltimore, 1972).
Cooperstein, S. J. & Watkins, D. (eds) The Islets of Langerhans. Biochemistry, Physiology, and Pathology (Academic, New York, 1981).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Villa-Komaroff, L. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3727–3731 (1978).
Lomedico, P. et al. Cell 18, 545–558 (1979).
Cordell, B. et al. Cell 18, 533–543 (1979).
Ullrich, A. et al. Science 204, 612–615 (1980).
Bell, G. I. et al. Nature 284, 26–32 (1980).
Wu, C. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 78, 1577–1580 (1981).
Kakita, K., Goddings, S. & Permutt, M. Proc. nat. Acad. Sci. U.S.A. 79, 2803–2807 (1982).
Walker, M., Edlund, T., Boulet, A. M. & Rutter, W. J. Nature 306, 557 (1983).
Cate, R., Chick, W. & Gilbert, W. J. biol. Chem. 258, 6645–6652 (1983).
Episkopou, V., Murphy, A. & Efstratiadis, A. Proc. natn. Acad. Sci. U.S.A. 81, 4657–4661 (1984).
Palmiter, R., Chen, H. & Brinster, R. Cell 29, 701–710 (1982).
Palmiter, R. et al. Nature 300, 615 (1982).
Lacy, E., Roberts, S., Evans, E. P., Burtenshaw, M. D. & Costantini, F. D. Cell 34, 343–358 (1983).
McKnight, G., Hammer, R. E., Kuenzel, E. A. & Brinster, R. L. Cell 34, 335–341 (1983).
Brinster, R. et al. Nature 306, 332 (1983).
Brinster, R. et al. Cell 37, 367–379 (1984).
Stewart, T., Pattengale, P. & Leder, P. Cell 38, 627–637 (1984).
Grosschedl, R., Weaver, D., Baltimore, D. & Costantini, F. Cell 38, 647–658 (1984).
Swift, G., Hammer, R. E., MacDonald, R. J. & Brinster, R. L. Cell 38, 639–646 (1984).
Chada, K. et al. Nature 314, 377–380 (1985).
Tooze, J. ed. Molecular Biology of Tumor Viruses Pt 2 (Cold Spring Harbor Laboratory, New York, 1981).
Chick, W. L. et al. Proc. natn. Acad. Sci. U.S.A. 74, 628–632 (1977).
Gazdar, A. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3519–3523 (1980).
Hanahan, D. Nature 312, 503–504 (1984).
Banerji, J., Rusconi, S. & Schaffner, W. Cell 27, 299–308 (1981).
Moreau, P. et al. Nucleic Acids Res. 9, 6047–6068 (1981).
Raskin, P. in The Islets of Langerhans (eds Cooperstein, J. J. & Watkins, D.) 467–481 (Academic, New York, 1982).
Pictet, R. & Rutter, W. J. in Endocrine Pancreas. Handbook of Physiology Vol. 1 (eds Steiner, D. F. & Frienkel, N.) 25–66) (Williams & Wilkins, Baltimore, 1972).
Pearse, A. C. E. Nature 295, 96–97 (1982).
Hargis, B. J. & Malkiel, S. J. nat. Cancer Inst. 63, 965–967 (1979).
Abramczuk, J., Pan, S., Maul, G. & Knowles, B. B. J. Virol. 49, 540–548 (1984).
Lewis, A. M. & Look, J. L. Science 227, 15–20 (1985).
Land, H., Parada, L. F. & Weinberg, R. A. Nature 304, 596–609 (1983).
Ruley, H. E. Nature 304, 602–606 (1983).
Folkman, J., Merler, E., Abernathy, C. & Williams, G. J. exp. Med. 133, 275–288 (1971).
Towbin, H., Staehelin, T. & Gordon, J. Proc. natl. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
Burnette, W. N. Analyt Biochem. 112, 195–203 (1981).
Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. M. J. Virol. 39, 861–869 (1981).
Gluzman, Y. Cell 23, 175–182 (1981).
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Hanahan, D. Heritable formation of pancreatic β-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315, 115–122 (1985). https://doi.org/10.1038/315115a0
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DOI: https://doi.org/10.1038/315115a0
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