Skip to main content
SpringerLink
Log in

Expression of Envelope Proteins of Endogeneous C-type Retrovirus on the Surface of Mouse and Human Oocytes at Fertilization

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Retrovirus genomes express, among other products, the envelop (env) proteins SU (gp70) and TM (p15E). They coexist at the viral surface membrane and are able to promote immunosuppression and membrane fusion. In mouse oocytes, endogeneous retroviruses (ERV) genomes are expressed at fertilization, and antigen epitopes of the Moloney murine leukemia virus (MuLV) env protein gp70 are recognized in the cytoplasm of the oocytes before but not after fertilization. By using a panel of monoclonal antibodies (mAbs) raised against env components, we checked with laser scanning confocal microscopy (LSCM) whether gp70 and p15E were expressed also on the oocyte surface membrane (oolemma). Since we found that both mouse and human unfertilized oocytes expressed these ERV proteins on the oolemma and that the expression enfeebled significantly after fertilization, we assume that ERV genomes could play a ro^le at the sperm-egg binding and fusion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mace M.C., Hansen M., Whiting S., Wang C.-T., and Barklis E., Virology 188, 869-874, 1992.

    Google Scholar 

  2. Larsson E., Kato N., and Cohen M., Curr Top Microbiol Immunol 148, 115-131, 1989.

    Google Scholar 

  3. Leib-MoÈsch C., Bachmann M., Brack-Wemer R., Wemer T., Er¯e V., and Hehlmann R., Leukemia 6, Suppl 3, 72S-75S, 1992.

    Google Scholar 

  4. Lyden T.W., Johnson P.M., Mwenda J.M., and Rote N.S., Biol Reprod 51, 152-157, 1994.

    Google Scholar 

  5. KaÈttstroÈm P.-O., Bjerneroth G., Nilsson B.O., Holmdahl R., and Larsson E., Cell Diff Develop 28, 47-54, 1989.

    Google Scholar 

  6. Larsson E., Nilsson B.O., SundstroÈm P., and WideÂhn S., Int J Cancer 28, 551-557, 1981.

    Google Scholar 

  7. Nilsson B.O., Larsson E., SundstroÈm P., and WideÂhn S., Upsala J Med Sci 86, 225-232, 1981.

    Google Scholar 

  8. Nilsson B.O., Larsson E., and SundstroÈm P., J In Vitro Fert Embryo Transfer 3, 296-303, 1986.

    Google Scholar 

  9. Cohen M., Powers M., O'Connell C., and Kato N., Virology 147, 449-458, 1985.

    Google Scholar 

  10. Venables P.J., Brookes S.M., Grif®ths D., Weiss R.A., and Boyd M.T., Virology 20, 589-592, 1995.

    Google Scholar 

  11. White J.M., Annu Rev Physiol 52, 675-697, 1990.

    Google Scholar 

  12. White J.M., Science 258, 917-924, 1992.

    Google Scholar 

  13. Fass D. and Kim P.S., Curr Biol 5, 1377-1383, 1995.

    Google Scholar 

  14. Kato N., Pfeifer-Ohlsson S., Kato M., Larsson E., Rydnert J., Ohlsson R., and Cohen M., Virology 61, 2182-2191, 1987.

    Google Scholar 

  15. Harris J.R., BioEssays 20, 307-316, 1998.

    Google Scholar 

  16. Larsson E., Andersson A.-C., and Nilsson B.O., Upsala J Med Sci 99, 113-120, 1994.

    Google Scholar 

  17. Larsson E., Suni J., Vaheri A., SundstroÈm P., and Nilsson O., Ann NY Acad Sci 442, 265-268, 1985.

    Google Scholar 

  18. Nilsson B.O., KaÈttstroÈm P.-O., SundstroÈm P., Jaquemin P., and Larsson E., Virus Genes 6, 221-227, 1992.

    Google Scholar 

  19. Myles D., Dev Biol 158, 35-45, 1993.

    Google Scholar 

  20. Green D.P.L., Hum Reprod 8, 91-96, 1993.

    Google Scholar 

  21. Hynes R.O., Cell 69, 11-25, 1992.

    Google Scholar 

  22. Preissner K.T., Ann Rev Cell Biol 7, 275-310, 1991.

    Google Scholar 

  23. Snell W.J. and White J.M., Cell 85, 629-637, 1996.

    Google Scholar 

  24. Blobel C.P., Wolfsbeerg T.G., Turck C.W., Myles D.G., Primakoff P., and White J.M., Nature 356, 248-252, 1992.

    Google Scholar 

  25. Bronson R.A. and Fusi F., Mol Hum Reprod 2, 153-168, 1996.

    Google Scholar 

  26. Fusi F.M., Bernocchi N., Ferrari A., and Bronson R.A., Mol Hum Reprod 2, 859-866, 1996.

    Google Scholar 

  27. Evans J.P., Schultz R.M., and Kaopf G.S., J Cell Sci 108, 3267-3278, 1995.

    Google Scholar 

  28. Primakoff P., Hyatt H., and Tredick-Kline J., J Cell Biol 104, 141-149, 1987.

    Google Scholar 

  29. Lincoln D.W., Br Med Bull 49, 222-236, 1993.

    Google Scholar 

  30. Kelly R.W., Hum Reprod 10, 1686-1693, 1995.

    Google Scholar 

  31. Wolf D.P. and Hamada M., J Cell Biol 96, 1786-1790, 1983.

    Google Scholar 

  32. Cohen D.J., Munuce M.J., and Cuasnicu P.S., Biol Reprod 55, 200-206, 1996.

    Google Scholar 

  33. Wolf D.P. and Hamada M., Biol Reprod 21, 205-211, 1979.

    Google Scholar 

  34. Jin M., Larsson A., and Nilsson B.O., Mol Reprod Dev 43, 47-54, 1996.

    Google Scholar 

  35. Lang M.S., Oostendorp A.J., Simons P.J., Boersma W., Knegt P., and van Ewijk W., Cancer Res 54, 1831-1836, 1994.

    Google Scholar 

  36. Cianciolo G.J., Phipps D., and Snyderman R., J Exp Med 159, 964-969, 1984.

    Google Scholar 

  37. Gabel C.A., Eddy E.M., and Shapiro B.M., Cell 18, 207-215, 1979.

    Google Scholar 

  38. Gaunt S.J., J Embryol Exp Morphol 75, 259-270, 1983.

    Google Scholar 

  39. Rein A., Mirro J., Haynes J.G., Ernst S., and Nagashima K., Virology 68, 1773-1781, 1994.

    Google Scholar 

  40. Wolf D.P., Dev Biol 64, 1-10, 1978.

    Google Scholar 

  41. Wolf D.P., ``The mammalian egg's block to polyspermy.'' In: Fertilization and embryologic development In Vitro. (Mastroianni Jr L., Bigger J.D.) Plenum Press, New York, 1981, 183-196.

    Google Scholar 

  42. Maleszewski M. and Bielak A., Zygote 1, 237-242, 1993.

    Google Scholar 

  43. Sengoku K., Tamate K., Horikawa M., Takaoka Y., Ishikawa M., and Dukelow W.R., J Reprod Fertil 105, 85-90, 1995.

    Google Scholar 

  44. Zuccotti M., Yanagimachi R., and Yanagimachi H., Development 112, 143-152, 1991.

    Google Scholar 

  45. Yanagimachi R., ``Sperm-egg fusion.'' In: Current Topics in Membranes and Transport. Vol. 32. (Duzgunes N., Bronner F.) Academic Press, Orlando, 1988, 3-43.

    Google Scholar 

  46. Sattentau Q.J. and Weiss R.A., Cell 52, 631-633, 1988.

    Google Scholar 

  47. Dorig R.E., Marcil A., Chopra A., and Richardson C.D., Cell 75, 295-305, 1993.

    Google Scholar 

  48. Bates P., Young J.A., and Varmus H.E., Cell 74, 1043-1051, 1993.

    Google Scholar 

  49. O'Rand M., Gamete Res 19, 315-328, 1988.

    Google Scholar 

  50. Hanada A. and Chang M.C., Biol Reprod 6, 300-309, 1972.

    Google Scholar 

  51. Maleszewski M., Kline D., and Yanagimachi R., J Reprod Fertil 105, 99-107, 1995.

    Google Scholar 

  52. Hanada A. and Chang M.C., J Exp Zool 203, 277-286, 1978.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, Biomedical Center, Box 571, S-751 23, Uppsala, Sweden

    B. Ove Nilsson & Meishan Jin

  2. Department of Pathology, Academic Hospital, S-751 85, Uppsala, Sweden

    Ann-Catrin Andersson & Erik Larsson

  3. Cura Clinic, Box 20037, S-200 74, Malmo¨, Sweden

    Per Sundstro¨m

Authors
  1. B. Ove Nilsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meishan Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ann-Catrin Andersson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Per Sundstro¨m
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Erik Larsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nilsson, B.O., Jin, M., Andersson, AC. et al. Expression of Envelope Proteins of Endogeneous C-type Retrovirus on the Surface of Mouse and Human Oocytes at Fertilization. Virus Genes 18, 115–120 (1999). https://doi.org/10.1023/A:1008004332513

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008004332513

Search

Navigation