Oral and maxillofacial surgeryControlled release of platelet growth factors enhances bone regeneration at rabbit calvaria
Section snippets
Preparation of gelatin hydrogels
A gelatin with an IEP of 5.0 was supplied by Nitta Gelatin Co., Osaka, Japan. All chemicals were purchased from Wako Pure Chemical Industries Osaka, Japan and used without further purification.
Gelatin hydrogels were prepared by the glutaraldehyde cross-linking of gelatin as reported previously.12 The water content of gelatin hydrogel (the weight ratio of water present in the hydrogel to the wet hydrogel) was 98 wt%. The resulting freeze-dried gelatin hydrogel was cut into a disk shape (5 mm in
Microfocus computed tomography evaluation
Fig. 2 shows the microfocus computed tomography image of bone defects 8 weeks after treatment with the gelatin hydrogel incorporating PRP or other agents. When the bone defect was treated with the gelatin hydrogel incorporating PRP (Fig. 2, A), complete bone regeneration at the defect was observed. On the other hand, incomplete bone regeneration was observed for the PRP-activated thrombin (Fig. 2, B). No bone regeneration was detected for the empty gelatin hydrogel and the untreated defect (
Discussion
The present study used 5-mm calvaria defects to evaluate bone regeneration in adult rabbits (approximately 20 weeks old). There has been much debate in the literature regarding the appropriate size of the defects for bone regeneration in calvaria models, and defects that do not heal spontaneously have been termed critical size defects. Although the 5-mm defect used in this study is smaller than the critical size defect of 15 mm recommended for rabbits,17 healing was still incomplete without any
Conclusion
For clinical application of platelet growth factors in PRP, one of the important technologies required is the controlled release of growth factors at the necessary site over an extended time period. This study indicates that the gelatin hydrogel is a promising biomaterial for the enhancement of bone regeneration by PRP platelet growth factors.
References (25)
- et al.
Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery
J Oral Maxillofac Surg
(1997) - et al.
Platelet-rich plasma: growth factor enhancement for bone grafts
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
(1998) Platelet-rich plasma: evidence to support its use
J Oral Maxillofac Surg
(2004)- et al.
Investigation of platelet-rich plasma in rabbit cranial defects: a pilot study
J Oral Maxillofac Surg
(2002) - et al.
Platelet-rich plasma: ready or not?
J Oral Maxillofac Surg
(2004) - et al.
Collagenous matrices as release carriers of exogenous growth factors
Biomaterials
(2004) Significance of release technology in tissue engineering
Drug Discov Today
(2005)- et al.
Effect of poly DL-lactide–co-glycolide implants and xenogeneic bone matrix-derived growth factors on calvarial bone repair in the rabbit
Biomaterials
(1994) - et al.
Bone regeneration by transforming growth factor beta1 released from a biodegradable hydrogel
J Control Release
(2000) - et al.
Platelet rich plasma: biology and new technology
J Craniofac Surg
(2005)
Platelet-rich plasma and bone graft materials: a review and a standardized research protocol
Implant Dent
Sinus grafting using platelet-rich plasma–initial case presentation
Pract Periodontics Aesthet Dent
Cited by (63)
Efficacy of Gelatin Hydrogel Impregnated with Concentrated Platelet Lysate in Murine Wound Healing
2019, Journal of Surgical ResearchA perspective on the physical, mechanical and biological specifications of bioinks and the development of functional tissues in 3D bioprinting
2018, BioprintingCitation Excerpt :However, several factors within the larger superfamilies have been identified as having substantial effects on cell behavior, such as differentiation and ECM synthesis [111–113] with BMP-2 and transforming growth factor beta (TGF-β), migration [114,115] and proliferation [116,117] with fibroblastic growth factor 2 (FGF-2) and epithelial growth factor (EGF)). The three most widely utilized techniques for the presentation of growth and morphogenetic factors to cells within a scaffold are media supplementation [118], controlled release [119,120] and conjugation. Supplementation can be utilized to systematically change the composition and concentrations of growth factors in the cell media.
Local application of gelatin hydrogel sheets impregnated with platelet-derived growth factor BB promotes tendon-to-bone healing after rotator cuff repair in rats
2015, Arthroscopy - Journal of Arthroscopic and Related SurgeryMicro-computed tomography and histomorphometric analysis of the effects of platelet-rich fibrin on bone regeneration in the rabbit calvarium
2015, Archives of Oral BiologyCitation Excerpt :Defects of different dimensions have been used in studies performed on rabbit calvarium. In the literature, although calvarial defects with a diameter of 15 mm are recommended for rabbit studies, defects of 10, 9, 8, 6.5 and 5 mm have also been used.13,15–19 In the studies performed, it was reported that defects measuring 5 mm in rabbit calvaria did not spontaneously recover within a period of 8 weeks.19,20