Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Oral and maxillofacial surgeryCompressive mechanical stress promotes osteoclast formation through RANKL expression on synovial cells
Section snippets
Primary rat synovial cells
Synovial tissues were obtained from the knee joints of male Sprague-Dawley rats (7 weeks old, 250∼350 g). All experiments followed the guidelines of the Intramural Animal Use and Care Committee of Kyushu Dental College. Synovial cells were isolated from the synovial tissues according to a method previously described.24 Briefly, the tissues were washed twice with α minimal essential medium (α-MEM) (Gibco-BRL, Gaithersburg, MD) and finely minced. The minced tissue samples were then mixed with 5
Expression of RANKL mRNA in synovial cells
After the synovial cells were cultured with or without PGE2 (10−6 mol/L) under the loading of compressive force (1.0 and 2.0 g/cm2) or application of uniaxial sinusoidal strain (10% or 20%), the changes in mRNA expression were examined by RT-PCR. As shown in Fig. 2, A, the expression of RANKL mRNA was detected under the condition of compressive force, with or without culturing with PGE2. In contrast, RANKL mRNA expression was not detected following the application of uniaxial sinusoidal strain,
Discussion
Bone and cartilage destruction in RA and TMJ disorders are partly mediated by proteinases, inflammatory cytokines, neuropeptides, and arachidonic acid catabolites.3, 12 Recently, the results of several studies have suggested that inflammatory osteoclastic bone resorption in the synovium plays an important role in bone destruction in RA.26, 27, 28 Several animal models of RA and TMJ disorders are currently available.29, 30 In the present study, we used the synovial cells from the knee joints to
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Effects of continuous and released compressive force on osteoclastogenesis in vitro
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2023, International Journal of Oral and Maxillofacial SurgeryCitation Excerpt :Nishio et al.28 indicated that among ADD patients, the anterior and posterior parts of the condyle exhibited the largest stress. On the other hand, Ichimiya et al.29 reported that static mechanical compression on the condylar head and disc synovial tissues can promote osteoclast formation through the up-regulation of RANKL expression in the synovial cells, leading to degenerative changes. Post-surgical MRI showed successful disc repositioning with bone regeneration around the condylar head, as evidenced by high signal around the original condyle cortical bone.
How is mechanobiology involved in bone regenerative medicine?
2022, Tissue and CellCitation Excerpt :Alternating compressive forces also regulate osteoblast differentiation in human PDLs and ATP secretion (Manokawinchoke et al., 2019). Continuous compressive force increases the differentiation of osteoclasts in the primary macrophages of mice and RAW264.7 cells (Hayakawa et al., 2015; Ichimiya et al., 2007; Ikeda et al., 2016).1 h after compression, a marked increase in osteoclast marker gene expression such as Nfatc1, Trap, Rank, Cathk, Cic7, Mmp9, Atp6i, Dcstamp, and Ocstamp is detected. Expression of these genes’ peaks at 3 h and then begins to decline (Hayakawa et al., 2015).
Mechanical loading and the control of stem cell behavior
2021, Archives of Oral BiologyCitation Excerpt :Mechanical force regulates RANKL and osteoprotegerin (OPG) expression in stromal cells, influencing osteoclast precursor cells. Continuous compressive force, but not cyclic tensile strain, stimulates RANKL expression in synovial fibroblasts (Ichimiya et al., 2007). Correspondingly, cyclic compressive stress generated by hydraulic pressure induces RANKL expression, in a magnitude-dependent manner (Jia et al., 2020).
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