Trends in Biotechnology
ReviewChondrogenesis and cartilage tissue engineering: the longer road to technology development
Section snippets
Cartilage tissue engineering: prospects and challenges
Articular cartilage has little capacity for self-repair but a relatively high incidence of damage and deterioration from common trauma such as sports injury and diseases such as osteoarthritis. Surgical procedures such as autologous chondrocyte implantation for cell-based repair of small chondral lesions, and subchondral bone drilling or microfracture to activate cartilage synthesis by progenitor cells, are practiced clinically. However, despite providing temporary relief from the symptoms of
Cartilage from differentiated chondrocytes
Cartilage tissue engineering using chondrocytes has been studied extensively and many three-dimensional scaffold and bioreactor culture systems have been developed. Chondrocytes are isolated from cartilage tissue, but useful quantities of healthy human cartilage from load-bearing joints are difficult to source because of the high risk of joint injury at the donor site. Consequently, most tissue engineering studies using differentiated chondrocytes have employed animal models or human fetal
Cartilage from mesenchymal stem cells
Ongoing ethical issues and immunorejection problems using embryonic stem cells and unresolved safety concerns about the tumorigenicity of embryonic and induced pluripotent stem cells [12] mean that tissue-derived mesenchymal stem cells represent the most practical stem cell type for cartilage tissue engineering. In particular, adipose-derived stem cells are an attractive resource for clinical applications. Adipose tissue is easy to access and in plentiful supply in most patients; mesenchymal
Other cell sources for cartilage production
It is possible that neither differentiated chondrocytes nor mesenchymal stem cells are the optimal starting cell type for cartilage tissue engineering. In vivo, these cells mediate normal wound responses and are responsible for the formation of scar tissue. Persistent expression of collagen type I and the production of fibrocartilage by cultured chondrocytes and chondroinduced stem cells could be a reflection of the role these cells play in healing damaged cartilage. Such wound responses are
Concluding remarks and future perspectives
Omics studies are enabling us to develop a deeper appreciation of the biochemical and physiological complexity of differentiation and tissue development. However, culture systems for producing functional tissues outside of the body have not yet been improved substantially by the current emphasis on investigating the molecular basis of stem cell differentiation. In many ways, this recent research has highlighted the extent of the difficulties affecting in vitro cartilage development.
The role of
Acknowledgments
Our work was funded by the Australian Research Council (ARC).
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