Original ContributionMass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease
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Reagents
SOD, xanthine oxidase, luminol, xanthine, and vitamins A, E, and C were purchased from Sigma–Aldrich Chemical (Milwaukee, WI, USA). HPLC-quality solvents, such as methanol, water, 2-propanol, hexane, and acetonitrile, were purchased from either Fisher Chemical (Phillipsburg, NJ, USA) or EM Science (Gibbstown, NJ, USA). Standards and deuterated standards of arachidonic acid including PGD2, E2, F2α, I2; TXB2; 5-HETE, 12-HETEs, 15-HETEs; HODEs; and EETs were purchased from Cayman Chemicals (Ann
ICP–MS-based ionomic profiling in saliva revealed significant alterations in redox status in saliva from periodontal patients
Compelling evidence suggests that periodontitis results from chronic inflammation and oxidative stress conditions caused by a loss of the delicate balance between host antioxidant defense and pro-oxidative factors. Furthermore, nutritional factors including antioxidant vitamins (vitamins C, A, and E) and minerals seem to play an important role in maintaining the balance of systematic redox status. We conducted an FFQ and 72-h diet recall to investigate the dietary patterns and antioxidant
Discussion
Periodontal diseases including gingivitis and periodontitis are the most prevalent inflammatory conditions in humans. Research conducted during the past decade has demonstrated that individual risk for periodontitis includes microbiological, genetic, and environmental factors, among which dietary habit plays an important role. Furthermore, it remains poorly understood how inflammatory response and alterations in local and systemic redox status under oxidative stress contribute to progression of
Acknowledgments
M.Z. acknowledges financial support of an A29 grant from the Shanghai Municipal Mission of Health and Family Planning (20114103). H.Y. acknowledges financial support of grants from the Ministry of Science and Technology of China (2012BAK01B00), National Key Basic Research Program of China (973 Program, No. 2012CB524900), National Natural Science Foundation of China (31170809), and Hundred Talents Program from the Chinese Academy of Sciences (2012OHTP07). We acknowledge the help in the SOD
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2020, Journal of Oral Biology and Craniofacial ResearchCitation Excerpt :In 2008, the term « Salivaomics» was proposed, which combines knowledge of various components in saliva, including genome, epigenome, transcriptome, proteome, metabolome, and microbiome.7,8 Numerous studies clearly show that the determination of biochemical parameters is informative when using saliva as a substrate.9–36 In particular, the important indicators are the acidity of the medium9,10 and the mineral composition of saliva.11
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These authors contributed equally to this work.