Elsevier

Fertility and Sterility

Volume 105, Issue 5, May 2016, Pages 1222-1227.e4
Fertility and Sterility

Original article
Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality

https://doi.org/10.1016/j.fertnstert.2016.01.011Get rights and content
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Objective

To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles.

Design

Retrospective study.

Setting

University-affiliated center.

Patient(s)

Women underwent PGD cycles of blastocyst biopsy and fluorescence in situ hybridization analysis.

Intervention(s)

Not applicable.

Main Outcome Measure(s)

Biopsied TE cell number of blastocysts, survival, and implantation rates.

Result(s)

The biopsied TE cell number was affected by the TE quality and experience of different embryologists. The diagnostic efficiency increased when from one to five cells were biopsied (86.7%, 91.7%%, 96.0%, 96.8%, to 98.7%) and was maximized when more than six cells were biopsied. To compare the clinical efficiencies, blastocysts were divided into four groups according to biopsied TE cell number: 1–5, 6–10, 11–15, and 16–41. For the blastocysts with grade A TE score, no significant difference was observed in the survival and implantation rates among the four groups. For the blastocysts with grades B and C TE scores, the survival rates showed no significant differences among the four groups, but a significant decreasing trend in implantation rates was observed with increasing biopsied TE cell number.

Conclusion(s)

The implantation potential is negatively affected by the biopsied TE cell number in blastocysts with poor TE morphological score.

Key Words

Blastocyst biopsy
biopsied cell number
trophectoderm quality
implantation

Cited by (0)

S.Z. has nothing to disclose. K.L. has nothing to disclose. D.C. has nothing to disclose. F.G. has nothing to disclose. Y.T. has nothing to disclose. C.L. has nothing to disclose. H.H. has nothing to disclose. Y.G. has nothing to disclose. G.L. has nothing to disclose. G.L. has nothing to disclose.

S.Z. and K.L. should be considered similar in author order.

Supported by grants from the Major State Basic Research Development Program of China (no. 2012CB944901), the National Science Foundation of China (nos. 81222007 and 81471510), and the Program for New Century Excellent Talents in University and the Research Fund of National Health and Family Planning Commission of China (no. 201402004).