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Rapid discrimination between Candida albicans and Candida dubliniensis by using real-time polymerase chain reaction

https://doi.org/10.1016/j.diagmicrobio.2007.01.015Get rights and content

Abstract

Several phenotypic methods have been used for the differentiation of Candida albicans and Candida dubliniensis, but molecular investigations are considered most reliable in their diagnostic value. Here, we suggest a rapid real-time polymerase chain reaction assay where the discrimination was achieved through melting point analysis with the help of the nonspecific fluorescent dye SybrGreen.

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Acknowledgments

The authors thank Ziauddin Khan (Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait), Radka Nikolova (Szent Laszlo Hospital for Infectious Diseases, Budapest, Hungary), and Robert Majoros (Department of Medical Microbiology, University of Debrecen, Hungary) for providing the C. dubliniensis strains for the study.

References (27)

  • S.F. Gee et al.

    Identification of four distinct genotypes of Candida dubliniensis and detection of microevolution in vitro and in vivo

    J. Clin. Microbiol.

    (2002)
  • J. Guarro et al.

    Developments in fungal taxonomy

    Clin. Microbiol. Rev.

    (1999)
  • M.C. Hsu et al.

    Species identification of medically important fungi by use of real-time LightCycler PCR

    J. Med. Microbiol.

    (2003)
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