AJOG ReviewsDiet-induced obesity in the rat: A model for gestational diabetes mellitus☆
Section snippets
Material and methods
The entire protocol was reviewed and approved by the local Ethical Committee for Animal Procedures (Katholieke Universiteit Leuven, Belgium).
Body weights and determination of parameters in blood (Table I and II)
After 4 weeks on either diet, CAF rats weighed significantly more than C rats (278.1±1.7 in CAF rats vs 250.8±1.3 in C rats, P<.0001). Four weeks on the cafeteria diet increased the rats' body weight by 31% compared with 16% for rats on the standard laboratory chow (Figure 1). Furthermore plasma glucose levels as well as plasma insulin and leptin concentrations were significantly increased in CAF rats (P<.01, P<.001, and P<.001, respectively; Table I). During pregnancy, CAF rats gained more
Comment
Gestational diabetes was achieved in this study by feeding pregnant rats a high-calorie “cafeteria” diet. This novel method of GDM development in rodents provides a very attractive model for the study of GDM, attractive because of its simplicity and because of its resemblance to GDM in women. It is associated with, and is likely to be initiated by, the most important risk factor for GDM in women (ie, obesity), an increasingly prevalent metabolic disorder of energy imbalance. The recent increase
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Supported by VIS grant No. VIS/00/008 from the Katholieke Universiteit Leuven, Belgium.