ReviewImaging meiotic spindles by polarization light microscopy: principles and applications to IVF
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Dr David Keefe serves as Medical Director of the combined IVF programmes at New England Medical Center in Boston, Massachusetts, and Women and Infants Hospital in Providence, Rhode Island. Dr Keefe also serves on the faculty as an Associate Professor at Tufts and Brown University Medical Schools. He graduated from Georgetown University School of Medicine and completed his residency in Obstetrics and Gynecology and fellowship in Reproductive Endocrinology/Infertility at Yale University School of
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The toxic effects and possible mechanisms of decabromodiphenyl ethane on mouse oocyte
2021, Ecotoxicology and Environmental SafetyCitation Excerpt :Spindle in meiotic cells is responsible for the separation of homologous chromosomes in meiosis I and sister chromatids in meiosis II, spindle assembly errors led to oocyte development retardation and aneuploidy (Howe and FitzHarris, 2013). Moreover, abnormal spindle morphology led to IVF failure and abnormal division (Keefe et al., 2003). Therefore, ROS-induced spindle defect inhibited maturation and fertilization of oocyte treated with DBDPE.
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2020, Molecular and Cellular EndocrinologyOocyte meiotic spindle morphology is a predictive marker of blastocyst ploidy—a prospective cohort study
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2019, Yen & Jaffe's Reproductive Endocrinology: Physiology, Pathophysiology, and Clinical Management: Eighth Edition
Dr David Keefe serves as Medical Director of the combined IVF programmes at New England Medical Center in Boston, Massachusetts, and Women and Infants Hospital in Providence, Rhode Island. Dr Keefe also serves on the faculty as an Associate Professor at Tufts and Brown University Medical Schools. He graduated from Georgetown University School of Medicine and completed his residency in Obstetrics and Gynecology and fellowship in Reproductive Endocrinology/Infertility at Yale University School of Medicine. He received research training through an NIH-funded grant in the Reproductive Biology Training Programme at Northwestern University and as the Kennedy–Dannreuther Research Fellow at Yale. Previously, he also trained in psychiatry at Harvard and University of Chicago. His research at Brown focuses on reproductive ageing in women and on the molecular and cellular basis of oocyte dysfunction. Before joining the Faculty at Brown, Dr Keefe was at Yale, where he directed the Oocyte Donation Programme and directed a laboratory funded by a Clinical Investigator Award from the NIH.
Paper based on a contribution presented at the Serono Symposium ‘Towards Optimizing ART: a Tribute to Howard and Georgeanna Jones’ in Williamsburg, Virginia, USA, April 2002.