Plasma catecholamines—analytical challenges and physiological limitations
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Baseline states of mind differentially affected diurnal salivary stress biomarkers: A preliminary study
2022, HeliyonCitation Excerpt :Regarding stress research, the HPA axis is the most widely studied system with cortisol being considered as a gold standard biomarker of stress [6]. On the other hand, catecholamines’ levels fluctuate a lot due to their short half-lives and various factors, so they are not very reliable indicators of stress [7, 8]. However, there are many surrogate markers of the SAM system, many of which are also present in the saliva.
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2018, TrAC - Trends in Analytical ChemistryCitation Excerpt :A consensual protocol for blood samples calls for centrifugation within 1 h of collection to make plasma [14]. Neurochemicals in plasma are stable at −20 °C for 6 weeks with sodium metabisulfite [17], and they are stable at −80 °C without any antioxidants for more than 6 years [18]. Thus, for long-term storage of urine samples, preserving them at −80 °C with acidification is recommended.
Increased fibrinogen responses to psychophysiological stress predict future endothelial dysfunction implications for cardiovascular disease?
2017, Brain, Behavior, and ImmunityCitation Excerpt :Measures of plasma adrenaline and noradrenaline were not assessed as venous measures of these hormones can be unreliable indicators of general activation of the sympathetic nervous system. In addition the half life of catecholamines in plasma is 1–2 min, and venous concentrations in the forearm have been shown to be primarily due to local muscle activity (Hjemdahl, 1993). In order to assess dynamic autonomic function more reliably would have required complex, invasive and costly techniques which were not included in this protocol.
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