Elsevier

Journal of Endodontics

Volume 11, Issue 8, August 1985, Pages 330-335
Journal of Endodontics

A radioimmunoassay determination of the concentrations of prostaglandins E2 and F in painful and asymptomatic human dental pulpsDeterminacion por radioinmunoensayo de las concentraciones de prostaglandinas E2 y F en pulpas dentales humanas sintomaticas y asintomaticas*,**,***

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The purpose of this study was to determine the concentrations of prostaglandin E2 and prostaglandin F in painful and asymptomatic human dental pulps. Pulps were obtained from three groups of teeth: uninflamed pulps, asymptomatic teeth with caries and/or large restorations, and symptomatic teeth with the clinical diagnosis of irreversible pulpitis. Pulps were dissected from the teeth and stored in liquid nitrogen. They were homogenized in 40% ethanol and a lipid solvent extraction was performed. Prostaglandin levels were measured using radioimmunoassay. The pulps which were likely to demonstrate inflammation had significantly higher mean concentrations of prostaglandin E2 than did the uninflamed pulps (p<0.05). Painful pulps had a much higher level of both prostaglandin E2 and prostaglandin F than the asymptomatic pulps (p < 0.01).

El propósito de este estudio fue determinar las concentraciones de PGE2 y PGF en pulpas humanas sintomáticas y asintomáticas. Las pulpas fueron obtenidas de tres grupos de dientes: pulpas sin inflamación; dientes asintomáticos con caries y/o grandes restauraciones y dientes sintomáticos con diagnóstico clínico de pulpitis irreversible. Las pulpas fueron disecadas y colocadas en nitrógeno líquido, luego homogeneizadas en etanol al 40%, y se realizó la extracción del solvente lipídico. Los niveles de prostaglandinas fueron medidos usando radioinmunoensayo. Las pulpas que demostraron tener inflamación tuvieron significativamente más alta concentración de PGE que las pulpas sin inflamación (p<0.05). Las pulpas sintomáticas tuvieron un nivel mucho más alto en ambas PGE2 y PGF que las pulpas asintomáticas (p<0.01).

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    *

    This study was supported by research funding from the Ohio Association of Endodontists.

    **

    The opinions and assertions are those of the authors and are not to be construed as official or as reflecting the views of the United States Air Force.

    ***

    This paper was adapted from a thesis submitted by Dr. Cohen in partial fulfillment of the requirements for the MS degree at the Ohio State University, Columbus, OH. A portion of this paper was presented at the 42nd Annual Session of the American Association of Endodontists, San Diego, Ca, and was honored by a graduate student research award.

    1

    Dr. Cohen is an endodontist with the United States Force and is stationed at McGuire AFB, NJ. Dr. Reader is associate professor, Department of Endodontics, The Ohio State University. Dr. Fertel is associate professor, Department of Pharmacology, College of Medicine, The Ohio State University. Dr. Beck is associate professor, Department of Oral Diagnosis and Medicine. The Ohio State University. Dr. Meyers is professor and chairman, Department of Endodontics, The Ohio State University

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