ORIGINAL ARTICLES: CARDIOVASCULARLeukocyte integrin expression in patients undergoing cardiopulmonary bypass
Section snippets
Patient groups
The protocol of the study was approved by our Ethical Committee and informed consent was obtained from all patients. Eighteen patients, undergoing primary elective coronary artery bypass grafting (CABG), were randomized into two groups in a double-blind fashion. One group (n = 8) received full-dose aprotinin [2] and the other group (n = 10) served as a control. Patients were excluded from the study if they met any of the following criteria: episodes of unstable angina or myocardial infarction
Intraoperative and postoperative characteristics of patients
There was no significant difference between aprotinin and placebo groups with regard to number of grafts, CPB time, and cross-clamp time (Table 1). All patients received internal thoracic artery graft to the left anterior descending artery. Patients in the aprotinin group lost significantly less blood through the chest drains and received significantly less blood transfusions at 12 hours postoperatively (Table 1). Patients in the two groups had a similarly low rate of postoperative
Comment
Neutrophil activation, adhesion to endothelium, and extravasation are decisive steps in the systemic inflammatory response. Activated neutrophils, along with their bactericidal effects, also may contribute significantly to injury of inflamed tissue [11]. Adhesion molecules play an important role during inflammation on both sides of the endothelial barrier, first by helping to recruit leukocytes from the vascular compartment to the vessel wall and subsequently by modulating their responsiveness
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Pathophysiology of cardiopulmonary bypass
2022, Cardiopulmonary Bypass: Advances in Extracorporeal Life SupportThe inflammatory effect of cardiopulmonary bypass on leukocyte extravasation in vivo
2008, Journal of Thoracic and Cardiovascular SurgeryCitation Excerpt :Flow cytometric analysis of blister leukocytes was performed immediately after centrifugation of blister samples, with all incubation (15 minutes) and washing steps being carried out in ice-cold phosphate-buffered saline, as previously described.13 Whole-blood samples were prepared for staining by using the Immunolyse whole-blood lysing technique (Beckman Coulter, Luton, United Kingdom), as previously described.23 Primary antibody incubations were carried out in phosphate-buffered saline at room temperature for 15 minutes, as per the manufacturer's recommendations.
Cardiopulmonary bypass
2007, PlateletsAprotinin improves cerebral protection: Evidence from a survival porcine model
2006, Journal of Thoracic and Cardiovascular SurgeryThe cellular immunological responses of patients undergoing coronary artery bypass grafting compared with those of patients undergoing valve replacement
2010, European Journal of Cardio-thoracic SurgeryThe safety of aprotinin and lysine-derived antifibrinolytic drugs in cardiac surgery: A meta-analysis
2009, CMAJ. Canadian Medical Association Journal