A culture chamber for the continuous biochemical and morphological study of living cells in tissue culture

https://doi.org/10.1016/0014-4827(53)90089-5Get rights and content

Abstract

A culture chamber has been described in which continuous phase contrast microscopic observation of growing tissue cultures can be made. The device meets the rigid requirements for optimum culture of tissues and for optical observation. The tissue can be exposed to biochemical reagents at will. Some of the observations made with this device have been described briefly.

References (3)

  • J.G. Carlson et al.

    Science

    (1947)
There are more references available in the full text version of this article.

Cited by (19)

  • Integrated optical molecular imaging system for four-dimensional real-time detection in living single cells

    2012, Biosensors and Bioelectronics
    Citation Excerpt :

    Live-sample imaging has increasingly become a method for cell morphology and structural characterization. Since the early 1950s, cell culture systems have been designed in parallel with improvements in microscopy and cell culture techniques for drug- or gene-delivery studies that require hours of long-term observation (Christiansen et al., 1953). In addition, a cell culture incubator called a “ringcubator” on an inverted microscope (Heidemann et al., 2003), and an “open-microincubator” format on an upright microscope (Picard et al., 2010) have been created.

  • Liver Cell-Based Therapy - Bioreactors as Enabling Technology

    2008, Principles of Regenerative Medicine
  • A multipurpose microperfusion chamber

    1970, Experimental Cell Research
View all citing articles on Scopus

This work was supported by a grant from the Damon Runyon Memorial Fund for Cancer Research.

View full text