Skip to main content

Advertisement

SpringerLink
Log in

Effect of cord blood serum on ex vivo human limbal epithelial cell culture

  • Published:
Journal of Ocular Biology, Diseases, and Informatics

Abstract

Limbal cell transplantation is an efficacious procedure for rehabilitation of visual acuity in patients with severe ocular surface disorders. Cultivation of limbal epithelial stem cell with fetal bovine serum for transplantation has been a promising treatment for reconstructing the ocular surface in severe limbal stem cell deficiency caused by Steven Johnson syndrome, chemical or thermal injury. This technique of “cell therapy” has been accepted worldwide but the cost of cultivating the cells for transplantation is high. The objective of this study was to investigate the effect of cord blood serum in place of fetal bovine serum on the growth of human limbal epithelial cell culture. Our group has experimented with human cord blood serum which was obtained free of cost from willing donors. The use of human cord blood serum in place of fetal bovine serum for ex vivo culture of limbal stem cell has helped us in reducing the cost of culture. Fresh human limbal tissues from donor cadavers were cultured on intact and denuded amniotic membrane. Cells were proliferated in vitro with cell culture media containing human cord blood serum. Reverse transcription-polymerase chain reaction and immunofluorescence cytochemistry of cultured human limbal epithelial stem cell was done for characterization of the cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Dua HS, Azuara-Blanco A. Limbal stem cell of the corneal epithelium. Surv Ophthalmol. 2000;44:415–24.

    Article  PubMed  CAS  Google Scholar 

  2. Ono K, Yokoo S, Mimura T, Usui T, Miyata K, Amano S. Autologous transplantation of conjunctival epithelial cell cultured on amniotic membrane in a rabbit model. Mol Vis. 2007;13:1138–43.

    PubMed  Google Scholar 

  3. Sangwan VS, Matalia HP, Vemuganti GK, Fatima A, Ifthekar G, Singh S. Clinical outcomes of autologous cultivated limbal epithelium transplantation. Indian J Ophthalmol. 2006;54:29–34.

    Article  PubMed  Google Scholar 

  4. Tsubota K, Satake Y, Kaido M, Shinozaki N, Shimura S, Bissen-Miyajima H, et al. Treatment of severe ocular surface disorders with corneal epithelial stem cell transplantation. N Engl J Med. 1999;340:1697–703.

    Article  PubMed  CAS  Google Scholar 

  5. Leonard P, Nakamura T, Koizumi N, Inatomi T, Sotozono C, Yokoi N. Autologous serum-derived cultivated oral epithelial transplants for severe ocular surface disease. Arch Ophthalmol. 2006;124:1543–51.

    Article  Google Scholar 

  6. Zhang X, Sun H, Tang X, Ji J, Li X, Sun J. Comparison of cell-suspension and explant culture of rabbit limbal epithelial cells. Exp Eye Res. 2005;80:227–33.

    Article  PubMed  CAS  Google Scholar 

  7. Koizumi N, Fullwood NJ, Bairaktaris G, Inatomi T, Kinoshita S, Quantock AJ. Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane. Invest Ophthalmol Vis Sci. 2000;41:2506–13.

    PubMed  CAS  Google Scholar 

  8. Kim JC, Tseng SCG. Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea. 1995;14:473–84.

    Article  PubMed  CAS  Google Scholar 

  9. Chen Z, de Paiva CS, Luo L, Kretzer FL, Pflugfelder SC, Li DQ. Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells. 2004;22:355–66.

    Article  PubMed  Google Scholar 

  10. Lindberg K, Brown ME, Chaves HV, Kenyon KR, Rheinwald JG. In vitro propagation of human ocular surface epithelial cells for transplantation. Invest Ophthalmol Vis Sci. 1993;34:2672–9.

    PubMed  CAS  Google Scholar 

  11. Talbot M, Carrier P, Giasson CJ, Deschambeault A, Guérin SL, Auger FA, et al. Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction. Mol Vis. 2006;12:65–75.

    PubMed  CAS  Google Scholar 

  12. Hassan N, Habibollah P, Masoumeh J, Abolhassan A, Jalal G, Seifalian AM. Properties of the amniotic membrane for potential use in tissue engineering. J Euro Cells Mater. 2008;15:88–99.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Institute of Post Graduate Medical Education And Research (IPGME&R), 244 A.J.C Bose Road, Kolkata, 700 020, West Bengal, India

    Jayanta Dutta

  2. Neurobiology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata, 700032, India

    Anindita Chakraborty & Sumantra Das

  3. Himadri Datta, Professor, Regional Institute of Ophthalmology, Medical College and Hospital, 88 College street, Kolkata, West Bengal, India

    Himadri Datta

Authors
  1. Anindita Chakraborty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jayanta Dutta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sumantra Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Himadri Datta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jayanta Dutta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chakraborty, A., Dutta, J., Das, S. et al. Effect of cord blood serum on ex vivo human limbal epithelial cell culture. j ocul biol dis inform 5, 77–82 (2012). https://doi.org/10.1007/s12177-013-9106-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12177-013-9106-5

Keywords

Search

Navigation