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Bmp 2 and Bmp 7 Induce Odonto- And Osteogenesis of Human Tooth Germ Stem Cells

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Abstract

Bone morphogenetic proteins (BMPs) initiate, promote, and maintain odontogenesis and osteogenesis. In this study, we studied the effect of bone morphogenic protein 2 (BMP 2) and bone morphogenic protein 7 (BMP 7) as differentiation inducers in tooth and bone regeneration. We compared the effect of BMP 2 and BMP 7 on odontogenic and osteogenic differentiation of human tooth germ stem cells (hTGSCs). Third molar-derived hTGSCs were characterized with mesenchymal stem cell surface markers by flow cytometry. BMP 2 and BMP 7 were transfected into hTGSCs and the cells were seeded onto six-well plates. One day after the transfection, hTGSCs were treated with odontogenic and osteogenic mediums for 14 days. For confirmation of odontogenic and osteogenic differentiation, mRNA levels of BMP2, BMP 7, collagen type 1 (COL1A), osteocalsin (OCN), and dentin sialophosphoprotein (DSPP) genes were measured by quantitative real-time PCR. In addition to this, immunocytochemistry was performed by odontogenic and osteogenic antibodies and mineralization obtained by von Kossa staining. Our results showed that the BMP 2 and BMP 7 both promoted odontogenic and osteogenic differentiation of hTGSCs. Data indicated that BMP 2 treatment and BMP 7 treatment induce odontogenic differentiation without affecting each other, whereas they induce osteogenic differentiation by triggering expression of each other. These findings provide a feasible tool for tooth and bone tissue engineering.

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Acknowledgments

We would like to thank Burcin Keskin for her help in flow-cytometry analysis. The authors deny any conflicts of interest.

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, 26 Ağustos Campus, Kayisdagi cad., Kayisdagi, Istanbul, TR-34755, Turkey

    P. Neslihan Taşlı, Safa Aydın & Fikrettin Şahin

  2. Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA

    Mehmet Emir Yalvaç

Authors
  1. P. Neslihan Taşlı
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  2. Safa Aydın
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  3. Mehmet Emir Yalvaç
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  4. Fikrettin Şahin
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Correspondence to Fikrettin Şahin.

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Taşlı, P.N., Aydın, S., Yalvaç, M.E. et al. Bmp 2 and Bmp 7 Induce Odonto- And Osteogenesis of Human Tooth Germ Stem Cells. Appl Biochem Biotechnol 172, 3016–3025 (2014). https://doi.org/10.1007/s12010-013-0706-0

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  • DOI: https://doi.org/10.1007/s12010-013-0706-0

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