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Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics

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Abstract

The ubiquitin–proteasome system facilitates the degradation of damaged proteins and regulators of growth and stress response. Alterations in this proteolytic system are associated with a variety of human pathologies. By restriction fragment differential display polymerase chain reaction (RFDD-PCR) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS) based on two-dimensional polyacrylamide gel electrophoresis (2-DE), differentially expressed genes and proteins of ubiquitin specific proteases (USPs), proteasome subuinits (PSs) and ubiquitin protein ligase E3A (UBE3A) were analyzed between breast cancer and adjacent normal tissues. Some of them were further verified as over-expression by immunohistochemical stain. Five genes of proteasome subunits (PSs), including PSMB5, PSMD1, PSMD2, PSMD8 and PSMD11, four genes of USPs, including USP9X, USP9Y, USP10 and USP25, and ubiquitin protein ligase E3A (UBE3A) were over-expressed (>3-fold) in breast cancer tissue compared to adjacent normal tissue, and over-expression (>4-fold) of proteins of PSMA1 and SMT3A were observed in breast cancer tissue. PSMD8, PSMD11 and UBE3A were further verified as over-expression by immunohistochemical stain. The action of ubiquitin–proteasome system were obviously enhanced in breast cancer, and selectively intervention in action of ubiquitin–proteasome system may be a useful method of treating human breast cancer.

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Acknowledgements

We want to thank Dr. Hu Weimin and Prof. Zhengwei Yang for technical assistance.

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Authors and Affiliations

  1. Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Renmin South Road No. 17, Chengdu, China

    Shishan Deng, Hongying Zhou, Ruohong Xiong, Youguang Lu, Lihua Dong & Huijun Yang

  2. The Affiliated Hospital, North Sichuan Medical College, Nanchong, China

    Dazhong Yan & Tianyong Xing

  3. Immunology and Molecular Biology Institute, North Sichuan Medical College, Nanchong, China

    Enjie Tang

  4. Department of Preclinical Medicine, Chengdu Medical College, Chengdu, China

    Huijun Yang

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  1. Shishan Deng
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  2. Hongying Zhou
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Correspondence to Huijun Yang.

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Deng, S., Zhou, H., Xiong, R. et al. Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics. Breast Cancer Res Treat 104, 21–30 (2007). https://doi.org/10.1007/s10549-006-9393-7

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  • DOI: https://doi.org/10.1007/s10549-006-9393-7

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