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Evaluation of Local CYP17A1 and CYP19A1 Expression Levels as Prognostic Factors in Postmenopausal Invasive Ductal Breast Cancer Cases

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Abstract

There is growing attention focused on local estrogen production in the breast tissue and its possible role in breast cancer initiation and progression. Understanding the underlying mechanisms for estrogen synthesis and the microenvironment consisting of tumor and its surrounding adipose tissue might open new avenues in breast cancer prevention, prognosis and treatment. In order to obtain insight, we compared peritumoral and tumor tissue expressions of CYP17A1 and CYP19A1 genes, which play an important role in estrogen biosynthesis. The paired tissue samples of 20 postmenopausal ER+/PR+ patients diagnosed with invasive ductal breast cancer were studied. In addition, 12 breast tissue samples obtained from premenopausal women without a history of breast cancer were also investigated as representative of normal conditions. Peritumoral adipose tissues expressed CYP19A1 approximately threefold higher than tumor itself (p = 0.001). A nonsignificant trend toward low expression of CYP17A1 was observed in peritumoral compared to tumor tissue (p = 0.687). Clinicopathological parameters and patient characteristics which are accepted as risk factors for breast cancer were also associated with individual and combined expressions of CYP17A1 and CYP19A1. This study offers that evaluation of CYP17A1 and CYP19A1 local expression levels might be useful for deciding on personalized treatment approaches and more accurate diagnosis, when evaluated together with several clinicopathological and disease risk factors. Considering the key role of these CYPs in estrogen synthesis, determining their expression levels may be useful as a postdiagnostic marker and for choosing the right treatment method in addition to the conventional approach.

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Funding

This study has been supported by The Scientific Research Projects Coordination Unit of Istanbul Technical University (No 36004).

Author Contributions

This study was designed, directed, and coordinated by Mete Bora Tüzüner and Oğuz Öztürk. Oğuz Öztürk, as the principal investigator, provided conceptual and technical guidance for all aspects of the research. Hakan Bermek suggested and commented on the design of the experiments. Hande Turna contributed to patient selection and data collection. Tülin Öztürk, Şennur İlvan, and Zerrin Calay performed and analyzed immunohistochemistry experiments and also contributed to specimen selection. Allison Pınar Eronat and Fatih Seyhan performed RNA extractions and cDNA constructions from the tissue samples. Mete Bora Tüzüner planned and performed qRT-PCR studies and analyzed the data with Halil İbrahim Kısakesen and Hülya Yılmaz Aydoğan. The manuscript was written by Mete Bora Tüzüner and Oğuz Öztürk and commented on by all authors.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey

    Mete Bora Tüzüner & Hakan Bermek

  2. Department of Pathology, Cerrahpasa Medical School, Istanbul, Turkey

    Tülin Öztürk, Zerrin Calay & Şennur İlvan

  3. Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Çapa, 34390, Istanbul, Turkey

    Allison Pınar Eronat, Fatih Seyhan, Halil İbrahim Kısakesen, Hülya Yılmaz-Aydoğan & Oğuz Öztürk

  4. Department of Internal Medicine, Section of Medical Oncology, Cerrahpasa Medical School, Istanbul, Turkey

    Hande Turna

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  1. Mete Bora Tüzüner
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Correspondence to Oğuz Öztürk.

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The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of this research.

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Tüzüner, M.B., Öztürk, T., Eronat, A.P. et al. Evaluation of Local CYP17A1 and CYP19A1 Expression Levels as Prognostic Factors in Postmenopausal Invasive Ductal Breast Cancer Cases. Biochem Genet 54, 784–802 (2016). https://doi.org/10.1007/s10528-016-9756-7

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  • DOI: https://doi.org/10.1007/s10528-016-9756-7

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