Skip to main content
Log in

Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cells

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Photodynamic therapy (PDT) is considered an effective alternative for the treatment of port-wine stains (PWS) using hemoporfin (hematoporphyrin monomethyl ether, HMME), a novel photosensitizer with better efficacy and lower recurrence. Vascular endothelial growth factor (VEGF) plays an important role in the development of PWS. Therefore, we conducted this study to investigate the effect of HMME-PDT on VEGF expression. Human vascular endothelial cells (HUVECs) were treated with different doses of HMME and irradiated with 410-nm light emitting-diode (LED) light. To assess cell viability, CCK-8 assays were performed. At 48 h after PDT, the expression of VEGF/VEGF receptor (VEGFR) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Measurement of VEGF protein was carried out using western blotting assays. Cell viability was significantly inhibited after HMME-PDT and was dose-dependent within a certain range. HMME-PDT decreased secretion of VEGF 48 h after irradiation in HUVECs as compared to controls. The downregulation of VEGF and VEGFR mRNA as well as VEGF protein expression was more significant in the high HMME concentration group (4 μg/mL) than in the lower concentration group (2 μg/mL). Our outcomes provide evidence, that HMME-PDT can downregulate VEGF expression in cultured HUVECs and may explain the efficacy of hemoporfin PDT for PWS treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Cordoro KM, Speetzen LS, Koerper MA, Frieden IJ (2009) Physiologic changes in vascular birthmarks during early infancy: mechanisms and clinical implications. J Am Acad Dermatol 60(4):669–675

    Article  PubMed  Google Scholar 

  2. Garzon MC, Huang JT, Enjolras O, Frieden IJ (2007) Vascular malformations: Part I. J Am Acad Dermatol 56:353–370

    Article  PubMed  Google Scholar 

  3. Klapman MH, Yao JF (2001) Thickening and nodules in port-wine stains. J Am Acad Dermatol 44:300–302

    Article  CAS  PubMed  Google Scholar 

  4. Hagen SL, Grey KR, Korta DZ, Kelly KM (2017) Quality of life in adults with facial port-wine stains. J Am Acad Dermatol 76:695–702

    Article  PubMed  Google Scholar 

  5. Brightman LA, Geronemus RG, Reddy KK (2015) Laser treatment of port-wine stains. Clin Cosmet Investig Dermatol 8(9):27

    PubMed  PubMed Central  Google Scholar 

  6. Asahina A, Watanabe T, Kishi A et al (2006) Evaluation of the treatment of port-wine stains with the 595-nm long pulsed dye laser: a large prospective study in adult Japanese patients. J Am Acad Dermatol 54:487–493

    Article  PubMed  Google Scholar 

  7. Gu Y, Li JH, Jiang YP, Liang L, Zhou P, Cui XJ, Pan YM, Wang K (1992) A clinical study of photodynamic therapy for port wine stain: a report of 40 cases. Chin J Laser Med Surg 1:81–84

    Google Scholar 

  8. Pu Y, Chen W, Yu Z (2012) Research progress of hemoporfin--part one: preclinical study. Photodiagn Photodyn Ther 9:180

    Article  CAS  Google Scholar 

  9. Yu W, Ma G, Qiu Y, Chen H, Jin Y, Yang X, Hu X, Chang L, Wang T, Zhou H, Li W, Lin X (2015) 18 years long-term results of facial port-wine stain (PWS) after photodynamic therapy (PDT)--a case report. Photodiagn Photodyn Ther 12:143–145

    Article  Google Scholar 

  10. Kowanetz M, Ferrara N (2006) Vascular endothelial growth factor signaling pathways: therapeutic perspective. Clin Cancer Res 12:5018–5022

    Article  CAS  PubMed  Google Scholar 

  11. Lei TC, Glazner GF, Duffy M, Scherrer L, Penyala S, Li B, Wang X, Wang H, Huang Z (2012) Optical properties of hematoporphyrin monomethyl ether (HMME), a PDT photosensitizer. Photodiagn Photodyn Ther 9:232–242

    Article  CAS  Google Scholar 

  12. Lu YG, Wu JJ, Yang YD, Yang YD, Yang HZ, He Y (2010) Photodynamic therapy of port-wine stains. J Dermatol Treat 21:240–244

    Article  CAS  Google Scholar 

  13. Ferrara N, Gerber HP, LeCouter J (2003) The biology of VEGF and its receptors. Nat Med 9:669–676

    Article  CAS  PubMed  Google Scholar 

  14. Otrock ZK, Makarem JA, Shamseddine AI (2007) Vascular endothelial growth factor family of ligands and receptors: review. Blood Cells Mol Dis 38:258–268

    Article  CAS  PubMed  Google Scholar 

  15. Kawczykkrupka A, Kwiatek B, Czuba Z, Mertas A, Latos W, Verwanger T, Krammer B, Sieron A (2018) Secretion of the angiogenic factor VEGF after photodynamic therapy with ALA under hypoxia-like conditions in colon cancer cells. Photodiagn Photodyn Ther 21:16–18

    Article  CAS  Google Scholar 

  16. Hera R, Keramidas M, Peoc’H M, Mouillon M, Romanet JP, Feige JJ (2005) Expression of VEGF and angiopoietins in subfoveal membranes from patients with age-related macular degeneration. Am J Ophthalmol 139:589–596

    Article  CAS  PubMed  Google Scholar 

  17. Moy WJ, Ma G, Kelly KM, Choi B (2016) Hemoporfin-mediated photodynamic therapy on normal vasculature: implications for phototherapy of port-wine stain birthmarks. J Clin Transl Res 2:107–111

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Vural E, Ramakrishnan J, Cetin N, Buckmiller L, Suen JY, Fan CY (2008) The expression of vascular endothelial growth factor and its receptors in port-wine stains. Otolaryngol Head Neck Surg 139:560–564

    Article  PubMed  Google Scholar 

Download references

Funding

The research was sponsored by the Natural Science Foundation of Shanghai.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhong Lu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Ethical Committee of Shanghai Huashan Hospital, Fudan University.

Informed consent

Informed consent was obtained from each participant.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ma, J., Lai, G. & Lu, Z. Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cells. Lasers Med Sci 34, 149–155 (2019). https://doi.org/10.1007/s10103-018-2649-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-018-2649-8

Keywords

Navigation