Abstract
Our aim was to analyze the prevalence of high-risk human papillomavirus (HR-HPV) and its association with risk factors related to cervical lesions. We used 362 cervical samples from a transversal study to detect nineteen types from the high-risk HPV clade by highly sensitive PCR. Unexpectedly, we found a very high prevalence of HPV type 66 (32.8%), particularly in low-grade squamous intraepithelial lesions. A significant association of HPV66 with previously sexually transmitted disease was observed (p < 0.05). Our results strongly suggest that HPV66 might be indicative of cervical lesions that will not progress to cancer. HPV genotyping by methods that grouped type 66 with other HR-HPV clade types should be interpreted with caution.
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Acknowledgements
We acknowledge the Cellular Pathology and Molecular Cancer Laboratory, UIMEO team, Oncology Hospital, CMN SXXI, IMSS, and the CONACyT “Manuscript Writing Training Team (CEMAI 2019-2)”.
Funding
This study was funded by National Council for Science and Technology Sectoral Funds (FOSISS) SALUD-2014-C01-234198. PROT/FIS/IMSS 1377, and partially funded by a grant to Dr. Alejandro García Carrancá from PAPIIT-UNAM (IN213016).
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Study conception and design: KJG, VPC, MMS, SVV. Data acquisition: KJG, VPC, SGR, SGG, XMV, RMM, RRR, PSS, PAS, PPA. Data analysis: KJG, VPC, SGG, AGC, JDME, RRR, SVV. Interpretation: KJG, VPC, SGG, RMM, AGC, JDME, MMS, SVV. Writing of the first draft: KJG, VPC, SGR, XMV, JDME, RRR, PSS, MMS, SVV. Critical revision of the article: AGC, JDME, RRR, PSS, PAS, PPA, MMS, SVV. Final approval of the final manuscript: AGC, JDME, MMS, SVV. All authors read and approved the final manuscript and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted under Ethics Committee number 2014-3505-6.
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Juárez-González, K., Paredes-Cervantes, V., Gordillo-Rodríguez, S. et al. High prevalence of human papillomavirus type 66 in low-grade cervical lesions of Mexican women. Arch Virol 165, 2633–2640 (2020). https://doi.org/10.1007/s00705-020-04771-8
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DOI: https://doi.org/10.1007/s00705-020-04771-8