Skip to main content

Advertisement

Log in

High prevalence of human papillomavirus type 66 in low-grade cervical lesions of Mexican women

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Our aim was to analyze the prevalence of high-risk human papillomavirus (HR-HPV) and its association with risk factors related to cervical lesions. We used 362 cervical samples from a transversal study to detect nineteen types from the high-risk HPV clade by highly sensitive PCR. Unexpectedly, we found a very high prevalence of HPV type 66 (32.8%), particularly in low-grade squamous intraepithelial lesions. A significant association of HPV66 with previously sexually transmitted disease was observed (p < 0.05). Our results strongly suggest that HPV66 might be indicative of cervical lesions that will not progress to cancer. HPV genotyping by methods that grouped type 66 with other HR-HPV clade types should be interpreted with caution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Secretaria de Salud S Información Estadística. In: gob.mx. https://www.gob.mx/salud/acciones-y-programas/informacion-estadistica.%2520Accessed%252029%2520Oct%25202019. Accessed 20 June 2020

  2. Arbyn M, Weiderpass E, Bruni L et al (2020) Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. Lancet Glob Health 8:e191–e203

    Article  PubMed  Google Scholar 

  3. Website. Bruni L, Albero G, Serrano B, et al (2019) Human papillomavirus and related diseases report. In: ICO/IARC Information Centre on HPV and Cancer (HPV Information Centre). https://www.hpvcentre.net/statistics/reports/XWX.pdf. Accessed 29 Oct 2019 (Human Papillomavirus and Related Diseases in the World. Accessed 20 June 2020

  4. Gheit T (2019) Mucosal and cutaneous human papillomavirus infections and cancer biology. Front Oncol 9:355

    Article  PubMed  PubMed Central  Google Scholar 

  5. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans (2012) Biological agents. Volume 100 B. A review of human carcinogens. IARC Monogr Eval Carcinog Risks Hum 100:1–441

    PubMed Central  Google Scholar 

  6. Bzhalava D, Eklund C, Dillner J (2015) International standardization and classification of human papillomavirus types. Virology 476:341–344

    Article  CAS  PubMed  Google Scholar 

  7. Muñoz N, Xavier Bosch F, de Sanjosé S et al (2003) Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med 348:518–527

    Article  PubMed  Google Scholar 

  8. de Villiers E-M, de Villiers E-M, Fauquet C et al (2004) Classification of papillomaviruses. Virology 324:17–27

    Article  PubMed  Google Scholar 

  9. Cogliano V, Baan R, Straif K et al (2005) Carcinogenicity of human papillomaviruses. Lancet Oncol 6:204

    Article  PubMed  Google Scholar 

  10. Serra IGSS, Araujo ED et al (2018) Prevalence of human papillomavirus types associated with cervical lesions in Sergipe state, Northeastern Brazil: high frequency of a possibly carcinogenic type. Epidemiol Infect 146:1184–1193

    Article  CAS  PubMed  Google Scholar 

  11. Tawheed AR, Beaudenon S, Favre M, Orth G (1991) Characterization of human papillomavirus type 66 from an invasive carcinoma of the uterine cervix. J Clin Microbiol 29:2656–2660

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Ouedraogo RA, Zohoncon TM, Guigma SP et al (2018) Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa. Papillomavirus Res 6:22–26

    Article  PubMed  PubMed Central  Google Scholar 

  13. Martora F, Della Pepa ME, Grimaldi E et al (2019) Seven years prevalence and distribution of high and low risk HPV genotypes in women living in the metropolitan area of Naples. Cancer Epidemiol 63:101625

    Article  PubMed  Google Scholar 

  14. Chen Z, de Freitas LB, Burk RD (2015) Evolution and classification of oncogenic human papillomavirus types and variants associated with cervical cancer. Methods Mol Biol 1249:3–26

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Hiller T (2006) Comparative analysis of 19 genital human papillomavirus types with regard to p53 degradation, immortalization, phylogeny, and epidemiologic risk classification. Cancer Epidemiol Biomark Prev 15:1262–1267

    Article  CAS  Google Scholar 

  16. Mansour M, Touka M, Hasan U et al (2007) E7 properties of mucosal human papillomavirus types 26, 53 and 66 correlate with their intermediate risk for cervical cancer development. Virology 367:1–9

    Article  CAS  PubMed  Google Scholar 

  17. Solomon D, Davey D, Kurman R et al (2002) The 2001 Bethesda System: Terminology for Reporting Results of Cervical Cytology. Obstet Gynecol Surv 57:505–507

    Article  Google Scholar 

  18. Website. https://www.who.int/reproductivehealth/publications/cancers/screening_and_treatment_of_precancerous_lesions/en/. Accessed 29 Oct 2019. Accessed 21 June 2020

  19. Xu L, Padalko E, Oštrbenk A et al (2018) Clinical evaluation of INNO-LiPA HPV genotyping EXTRA II assay using the VALGENT framework. Int J Mol Sci 19:2704

    Article  PubMed Central  Google Scholar 

  20. Talavera JO, Rivas-Ruiz R, Bernal-Rosales LP (2011) Investigación clínica V. Tamaño de muestra. Rev Med Inst Mex Seguro Soc 49:517–522

    PubMed  Google Scholar 

  21. Monsonego J, Pollini G, Evrard MJ et al (2008) Linear array genotyping and hybrid capture II assay in detecting human papillomavirus genotypes in women referred for colposcopy due to abnormal Papanicolaou smear. Int J STD AIDS 19:385–392

    Article  PubMed  Google Scholar 

  22. Ortega-Cervantes L, Aguilar-Lemarroy A, Rojas-García AE (2016) Human papilloma virus genotypes in women from Nayarit, Mexico with squamous intraepithelial lesions and cervical cancer. Int J Health Sci 10:309–320

    Article  Google Scholar 

  23. Flores-Miramontes MG, Torres-Reyes LA, Alvarado-Ruíz L et al (2015) Human papillomavirus genotyping by linear array and next-generation sequencing in cervical samples from Western Mexico. Virol J 12:1–11

    Article  Google Scholar 

  24. Chouhy D, D’Andrea RM, Iglesias M et al (2013) Prevalence of human papillomavirus infection in Argentinean women attending two different hospitals prior to the implementation of the National Vaccination Program. J Med Virol 85:655–666

    Article  PubMed  PubMed Central  Google Scholar 

  25. Saville M, Sultana F, Malloy MJ et al (2018) Clinical validation of the cobas HPV Test on the cobas 6800 system for the purpose of cervical screening. J Clin Microbiol. https://doi.org/10.1128/JCM.01239-18

  26. Cook DA, Mei W, Smith LW et al (2015) Comparison of the Roche cobas® 4800 and Digene Hybrid Capture® 2 HPV tests for primary cervical cancer screening in the HPV FOCAL trial. BMC Cancer. https://doi.org/10.1186/s12885-015-1959-5

  27. Dols JAM, Reid G, Kort R et al (2012) PCR-based identification of eight lactobacillus species and 18 hr-HPV genotypes in fixed cervical samples of south african women at risk of HIV and BV. Diagn Cytopathol 40:472–477

    Article  PubMed  Google Scholar 

  28. Teixeira MF, Sabidó M, Leturiondo AL et al (2018) High risk human papillomavirus prevalence and genotype distribution among women infected with HIV in Manaus, Amazonas. Virol J. https://doi.org/10.1186/s12985-018-0942-6

  29. Kim H-S, Kim TJ, Lee I-H, Hong SR (2016) Associations between sexually transmitted infections, high-risk human papillomavirus infection, and abnormal cervical Pap smear results in OB/GYN outpatients. J Gynecol Oncol. https://doi.org/10.3802/jgo.2016.27.e49

  30. Aguilar-Lemarroy A, Vallejo-Ruiz V, Cortés-Gutiérrez EI et al (2015) Human papillomavirus infections in Mexican women with normal cytology, precancerous lesions, and cervical cancer: Type-specific prevalence and HPV coinfections. J Med Virol 87:871–884

    Article  PubMed  Google Scholar 

  31. Salcedo M, Pina-Sanchez P, Vallejo-Ruiz V et al (2015) Human papillomavirus genotypes among females in Mexico: a study from the Mexican Institute for Social Security. Asian Pac J Cancer Prev 15:10061–10066

    Article  Google Scholar 

  32. DelaRosa-Martínez R, Sánchez-Garza M, López-Revilla R (2016) HPV genotype distribution and anomalous association of HPV33 to cervical neoplastic lesions in San Luis Potosí, Mexico. Infect Agents Cancer. https://doi.org/10.1186/s13027-016-0063-z

  33. López-Revilla R, Martínez-Contreras LA, Sánchez-Garza M (2008) Prevalence of high-risk human papillomavirus types in Mexican women with cervical intraepithelial neoplasia and invasive carcinoma. Infect Agents Cancer. https://doi.org/10.1186/1750-9378-3-3

  34. Guan P, Howell-Jones R, Li N et al (2012) Human papillomavirus types in 115,789 HPV-positive women: a meta-analysis from cervical infection to cancer. Int J Cancer 131:2349–2359

    Article  CAS  PubMed  Google Scholar 

  35. Kojic EM, Conley L, Bush T et al (2018) Prevalence and incidence of anal and cervical high-risk human papillomavirus (HPV) types covered by current HPV vaccines among HIV-infected women in the SUN study. J Infect Dis 217:1544–1552

    Article  PubMed  Google Scholar 

  36. Nielsen A, Iftner T, Munk C, Kjaer SK (2009) Acquisition of high-risk human papillomavirus infection in a population-based cohort of Danish Women. Sex Transm Dis 36:609–615

    Article  PubMed  PubMed Central  Google Scholar 

  37. Pannier-Stockman C, Segard C, Bennamar S et al (2008) Prevalence of HPV genotypes determined by PCR and DNA sequencing in cervical specimens from French women with or without abnormalities. J Clin Virol 42:353–360

    Article  CAS  PubMed  Google Scholar 

  38. Sasagawa T, Basha W, Yamazaki H, Inoue M (2001) High-risk and multiple human papillomavirus infections associated with cervical abnormalities in Japanese women. Cancer Epidemiol Biomark Prev 10:45–52

    CAS  Google Scholar 

  39. Elfström KM, Miriam Elfström K, Smelov V et al (2015) Long-term HPV type-specific risks for ASCUS and LSIL: a 14-year follow-up of a randomized primary HPV screening trial. Int J Cancer 136:350–359

    Article  PubMed  Google Scholar 

  40. Smelov V, Miriam Elfström K, Johansson ALV et al (2015) Long-term HPV type-specific risks of high-grade cervical intraepithelial lesions: a 14-year follow-up of a randomized primary HPV screening trial. Int J Cancer 136:1171–1180

    Article  CAS  PubMed  Google Scholar 

  41. Deacon JM, Evans CD, Yule R et al (2000) Sexual behaviour and smoking as determinants of cervical HPV infection and of CIN3 among those infected: a case–control study nested within the Manchester cohort. Br J Cancer 83:1565–1572

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We acknowledge the Cellular Pathology and Molecular Cancer Laboratory, UIMEO team, Oncology Hospital, CMN SXXI, IMSS, and the CONACyT “Manuscript Writing Training Team (CEMAI 2019-2)”.

Funding

This study was funded by National Council for Science and Technology Sectoral Funds (FOSISS) SALUD-2014-C01-234198. PROT/FIS/IMSS 1377, and partially funded by a grant to Dr. Alejandro García Carrancá from PAPIIT-UNAM (IN213016).

Author information

Authors and Affiliations

Authors

Contributions

Study conception and design: KJG, VPC, MMS, SVV. Data acquisition: KJG, VPC, SGR, SGG, XMV, RMM, RRR, PSS, PAS, PPA. Data analysis: KJG, VPC, SGG, AGC, JDME, RRR, SVV. Interpretation: KJG, VPC, SGG, RMM, AGC, JDME, MMS, SVV. Writing of the first draft: KJG, VPC, SGR, XMV, JDME, RRR, PSS, MMS, SVV. Critical revision of the article: AGC, JDME, RRR, PSS, PAS, PPA, MMS, SVV. Final approval of the final manuscript: AGC, JDME, MMS, SVV. All authors read and approved the final manuscript and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Corresponding authors

Correspondence to Martha Martínez-Salazar or Salvador Vázquez-Vega.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted under Ethics Committee number 2014-3505-6.

Consent to participate

Informed consent was obtained from all individual participants included in the study.

Consent for publication

Consent for publication was obtained from all individual participants included in the study.

Availability of data and material

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Handling Editor: Carolina Scagnolari.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Juárez-González, K., Paredes-Cervantes, V., Gordillo-Rodríguez, S. et al. High prevalence of human papillomavirus type 66 in low-grade cervical lesions of Mexican women. Arch Virol 165, 2633–2640 (2020). https://doi.org/10.1007/s00705-020-04771-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-020-04771-8

Navigation