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Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia

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Abstract

Purpose

Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.

Methods

Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.

Results

Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.

Conclusions

These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.

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Acknowledgments

The work was supported by National Natural Science Foundation (No. 81260123) and Nature Science Foundation of the Guangxi Zhuang Autonomous Region to Shulin Shen (No. Gui ke zi 0991271).

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Andrology, Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou, 545001, China

    Shulin Shen

  2. College of Life Science and Technology, Guangxi University, Nanning, 530004, China

    Jinzi Wang

  3. Department of Andrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530007, China

    Jihong Liang

  4. Department of Urology, The First Affiliated Hospital of Xian Jiao Tong University, Sian, 710061, China

    Dalin He

Authors
  1. Shulin Shen
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  2. Jinzi Wang
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  3. Jihong Liang
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  4. Dalin He
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Corresponding author

Correspondence to Dalin He.

Additional information

Shulin Shen and Jinzi Wang contributed equally to this work.

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Shen, S., Wang, J., Liang, J. et al. Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia. World J Urol 31, 1395–1401 (2013). https://doi.org/10.1007/s00345-013-1023-5

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  • DOI: https://doi.org/10.1007/s00345-013-1023-5

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