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Transcriptional response to ionizing radiation in lymphocyte subsets

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Abstract.

Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations [CD4+ and CD8+ T, CD19+ B, CD56+ natural killer cells and peripheral blood lymphocytes (PBLs)] from ten adult donors. In contrast to DDB2, TNFRSF10B and BAX were differentially modulated among the subpopulations and the PBLs, being more activated in irradiated CD19+ B and CD8+ T lymphocytes. The level of BAX activation in the various subpopulations correlated with the sensitivity of the cells to radiation, suggesting its possible role in the differential radiosensitivity of hematopoietic cell subsets.

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Correspondence to M. Mori.

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Received 3 March 2005; accepted 26 April 2005

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Mori, M., Benotmane, M.A., Tirone, I. et al. Transcriptional response to ionizing radiation in lymphocyte subsets. CMLS, Cell. Mol. Life Sci. 62, 1489–1501 (2005). https://doi.org/10.1007/s00018-005-5086-3

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  • DOI: https://doi.org/10.1007/s00018-005-5086-3

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