Abstract.
Objective:
Severe trauma leads to an increased vulnerability to bacterial sepsis. In the present study, we compared the immunostimulating potential of granulocyte-colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma (IFN-γ).
Design:
Prospective clinical experimental study.
Setting:
University hospital intensive care unit and research facility.
Patients:
6 Patients with an Injury Severity Score (ISS) of more than 25 points.
Interventions:
Heparinized blood samples of severely injured patients and 12 healthy volunteers were incubated in vitro with 10 ng/ml GM-CSF, 10 ng/ml G-CSF or 10 ng/ml IFN-γ for 6h.
Measurements:
Flow cytometry: HLA-DR expression on monocytes, B- and T-lymphocytes.
ELISA:
LPS-induced TNFα and IL-10 production.
Results:
In all patients reduced cytokine production and HLA-DR expression on monocytes was established. After administration of GM-CSF and IFN-γ it in vitro, the level of HLA-DR expression on monocytes and the it ex vivo TNFα-synthesis increased while only GM-CSF increased significantly IL-10-liberation after LPS-stimulation. However, only IFN-γ had the capacity to enhance HLA-DR on B- and T-Lymphocytes. G-CSF it in vitro had no significant effect on the measured parameter.
Conclusions:
These data suggest that GM-CSF and IFN-γ may serve to support immune functions in severely injured patients.
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Received 10 January 2006; returned for revision 15 June 2006; accepted by M. Parnham 9 August 2006
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Lendemans, S., Kreuzfelder, E., Waydhas, C. et al. Differential immunostimulating effect of Granulocyte-macrophage colony-stimulating factor (GM-CSF), Granulocyte colony-stimulating factor (G-CSF) and Interferon γ (IFNγ) after severe trauma. Inflamm. res. 56, 38–44 (2007). https://doi.org/10.1007/s00011-007-6069-7
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DOI: https://doi.org/10.1007/s00011-007-6069-7