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In vitro screening of crude extracts and pure metabolites obtained from marine invertebrates for the treatment of breast cancer

  • Original Articles
  • In Vitro Screening, Marine Organisms, Breast Cancer
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Summary

A total of 15 samples (crude extracts and pure secondary metabolites) obtained from marine invertebrates collected from the offshore waters of British Columbia, Papua New Guinea, and Sri Lanka have previously been shown to exert cytotoxic activity in the in vitro L1210 leukemic bioassay. We screened these metabolites for in vitro cytotoxic activity against the drug-sensitive breast-tumor cell lines MCF-7, T-47D, ZR-75-1, and MDA-MB-231; the multidrug-resistant and P-glycoprotein (Pgp)-positive breast-tumor cell lines MCF-7 Adr and MDA-Alr; and normal and malignant human breast epithelial cells (HBEC) in primary culture. Eight samples exhibited significant [drug concentration resulting in a 50% decrease in cell growth as compared with controls (ED50),<25 μg/ml] dose-dependent cytotoxicity against the drug-sensitive cell lines; the ED50 values were as low as 0.004 μg/ml. Five of the eight samples exhibited significant cytotoxicity against the multidrug-resistant cell lines; the ED50 values were as low as 0.0006 μg/ml. Incubation of MCF-7 Adr cells with varying concentrations of compounds in the presence of Adriamycin demonstrated that none of the compounds tested interfered with Pgp function. Results obtained using HBEC in primary culture showed a wide range of chemosensitivities for a given drug against tissue taken from different patients, demonstrating the uniqueness of the response of different individuals to chemotherapy.

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Authors and Affiliations

  1. Department of Chemistry, University of British Columbia, Vancouver, B. C., Canada

    Raymond J. Andersen

  2. Department of Anatomy, University of British Columbia, 2177 Wesbrook Mall, V6T 1Z3, Vancouver, B. C., Canada

    John Stingl & Joanne T. Emerman

Authors
  1. John Stingl
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  2. Raymond J. Andersen
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  3. Joanne T. Emerman
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Additional information

This work was supported by grants from the National Cancer Institute of Canada and the B. C. Health Research Foundation, and by funds donated by Ms. J. Harowitz

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Stingl, J., Andersen, R.J. & Emerman, J.T. In vitro screening of crude extracts and pure metabolites obtained from marine invertebrates for the treatment of breast cancer. Cancer Chemother. Pharmacol. 30, 401–406 (1992). https://doi.org/10.1007/BF00689969

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  • DOI: https://doi.org/10.1007/BF00689969

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