Abstract
Prostate cancer is the most common non-cutaneous cancers among men and the second leading cause of cancer-related deaths among men. Aberrant activation of the epithelial to mesenchymal transition (EMT) has been exhibited to be one of the most common causes of treatment failure and death in cancer patients. In cancer cells with metastatic competence, the E-cadherin switch is a well-established hallmark. Suppression of E-cadherin through its transcriptional repressor SNAIL is thus a determining factor for EMT. TWIST1 is an important transcription factor in EMT, which is present under both physiologic (embryogenesis) and pathologic (metastasis) conditions, and enhances the invasiveness and migration ability of cells. In this study, we investigated the inhibitory effects of taurine on EMT-related genes, such as E-cadherin, N-cadherin, TWIST1, ZEB1, SNAIL, and vimentin. EMT markers were detected by RT-PCR and western blotting. The results showed that taurine down-regulated the expression of N-cadherin, TWIST1, ZEB1, SNAIL, and vimentin. In contrast, taurine increased E-cadherin expression. Our findings indicate that taurine has EMT inhibitory effects on human prostate cancer cells.
Keywords
$These authors contributed equally to this work.
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- EMT:
-
Epithelial to mesenchymal transition
- PSA:
-
Prostate-specific antigen
- SNAIL:
-
Snail family zinc finger 1
- TWIST1:
-
Basic helix-loop-helix protein 38
- ZEB1:
-
Zinc finger E-box binding homeobox 1
References
Barrallo-Gimeno A, Nieto MA (2005) The Snail genes as inducers of cell movement and survival: implications in development and cancer. Development 132:3151–3161
El-Houseini ME, Refaei MO, Amin AI, Abol-Ftouh MA (2013) Potential role of curcumin and taurine combination therapy on human myeloid leukemic cells prop-agated in vitro. Leuk Lymphoma 54:2281–2287
Ferlay J, Steliarova-Foucher E, Lortet-Tieulent J, Rosso S, Coebergh JW, Comber H, Forman D, Bray F (2013) Cancer incidence and mortality patterns in Europe: estimates for 40 countries in 2012. Eur J Cancer 49:1374–1403
Fu J, Qin L, He T, Qin J, Hong J, Wong J, Liao L, Xu J (2011) The TWIST/Mi2/NuRD protein complex and its essential role in cancer metastasis. Cell Res 21:275–289
Fu W, Tao T, Qi M, Wang L, Hu J, Li X, Xing N, Du R, Han B (2016) MicroRNA-132/212 upregulation inhibits TGF-β-mediated epithelial-mesenchymal transition of prostate cancer cells by targeting SOX4. Prostate 76(16):1560–1570
Garg M (2013) Epithelial-mesenchymal transition - activating transcription factors—multifunctional regulators in cancer. World J Stem Cells 5:188–195
Ge A, Ma Y, Liu YN, Li YS, Gu H, Zhang JX, Wang QX, Zeng XN, Huang M (2016) Diosmetin prevents TGF-β1-induced epithelial-mesenchymal transition via ROS/MAPK signaling pathways. Life Sci 153:1–8
Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646–674
Kalluri R, Weinberg RA (2009) The basics of epithelial-mesenchymal transition. J Clin Invest 119:1420–1428
Kim SH, Kim EK, Choi EJ (2014) High-intensity swimming exercise increases dust mite extract and 1-chloro-2,4-dinitrobenzene-derived atopic dermatitis in BALB/c mice. Inflammation 37:1179–1185
Nieto MA (2002) The snail superfamily of zinc-finger transcription factors. Nat Rev Mol Cell Biol 3:155–166
Perez-Moreno MA, Locascio A, Rodrigo I, Dhondt G, Portillo F, Nieto MA, Cano A (2001) A new role for E12/E47 in the repression of E-cadherin expression and epithelial-mesen- chymal transitions. J Biol Chem 276:27424–27431
Qin Q, Xu Y, He T, Qin C, Xu J (2012) Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms. Cell Res 22:90–106
Rodriguez M, Aladowicz E, Lanfrancone L, Goding CR (2008) Tbx3 represses E-cadherin expression and enhances melanoma invasiveness. Cancer Res 68:7872–7881
Scarpa ES, Ninfali P (2015) Phytochemicals as innovative therapeutic tools against cancer stem cells. Int J Mol Sci 16:15727–15742
Scheel C, Eaton EN, Li SH, Chaffer CL, Reinhardt F, Kah KJ, Bell G, Guo W, Rubin J, Richardson AL, Weinberg RA (2011) Para-crine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell 145:926–940
Shankar S, Nall D, Tang SN, Meeker D, Passarini J, Sharma J, Srivastava RK (2011) Resveratrol inhibits pancreatic cancer stem cell characteristics in human and KrasG12D transgenic mice by inhibiting pluripotency maintaining factors and epithelial-mesenchymal transition. PLoS One 6:e16530
Tang Y, Jeon BT, Wang Y, Choi EJ, Kim YS, Hwang JW, Park PJ, Moon SH, Kim EK (2015) First evidence that Sika Deer (Cervus nippon) Velvet Antler extract suppresses migration of human prostate cancer cells. Korean J Food Sci Anim Resour 35:507–514
Thiery JP, Acloque H, Huang RY, Nieto MA (2009) Epithelial-mesenchymal transitions in development and disease. Cell 139:871–890
Tormey WP (2014) The complexity of PSA interpretation in clinical practice. J Coll Surg Edinb 12:323–327
Yang F, Sun L, Li Q, Han X, Lei L, Zhang H, Shang Y (2012) SET8 promotes epithelial-mesenchymal transition and confers TWIST dual transcriptional activities. EMBO J 31:110–123
Yang J, Mani SA, Donaher JL, Ramaswamy S, Itzykson RA, Come C, Savagner P, Gitelman I, Richardson A, Weinberg RA (2004) Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell 117:927–939
Yang MH, Wu MZ, Chiou SH, Chen PM, Chang SY, Liu CJ, Teng SC, Wu KJ (2008) Direct regulation of TWIST by HIF-1alpha promotes metastasis. Nat Cell Biol 10:295–305
Zhang X, Tu S, Wang Y, Xu B, Wan F (2014) Mechanism of taurine-induced apoptosis in human colon cancer cells. Acta Biochim Biophys Sin Shanghai 46:261–272
Acknowledgments
This research was supported by the Basic Science Research Program through the National Research Foundation of South Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A3006958), and Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Agri-Bio industry Technology Development Program (316027-5) funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA).
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Tang, Y. et al. (2017). Taurine Attenuates Epithelial-Mesenchymal Transition-Related Genes in Human Prostate Cancer Cells. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_96
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DOI: https://doi.org/10.1007/978-94-024-1079-2_96
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