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Tumor Microenvironment pp 145–166Cite as

Methods for Studying Autophagy Within the Tumor Microenvironment

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 899))

Abstract

Defective autophagy has been linked with many pathologies, including cancer, diabetes, infectious disease, myopathies, heart, liver, lung, and neurodegenerative disease. Autophagy has therefore become an important target in drug discovery. Recent advances have identified new ways to monitor autophagy in vitro and in vivo. Many assays rely on visualizing autophagy-related intracellular markers such as microtubule-associated protein light chain 3 (LC3) II, which have posed issues with in vivo and clinical translation of the in vitro assays. Here, we present an overview of current in vitro and in vivo methodologies to measure autophagy with a special focus on the tumor microenvironment.

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Author information

Authors and Affiliations

  1. Hematology/Oncology Division, Perelman School of Medicine, University of Pennsylvania, Clinical Research Building Room No: 120, Philadelphia, PA, 19104, USA

    Sengottuvelan Murugan Ph.D.

  2. The Department of Medicine and Abramson Cancer Center, University of Pennsylvania School of Medicine, 777 South Tower 34th St and Civic Center Blvd, Philadelphia, PA, 19104, USA

    Ravi K. Amaravadi M.D.

Authors
  1. Sengottuvelan Murugan Ph.D.
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  2. Ravi K. Amaravadi M.D.
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Corresponding author

Correspondence to Ravi K. Amaravadi M.D. .

Editor information

Editors and Affiliations

  1. Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Constantinos Koumenis

  2. Cell & Developmental Biology, Oregon Health & Science University, Portland, Oregon, USA

    Lisa M. Coussens

  3. Department of Radiation Oncology, Stanford University School of Medic, Stanford, California, USA

    Amato Giaccia

  4. Department of Oncology, University of Oxford, Oxford, Oxfordshire, United Kingdom

    Ester Hammond

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© 2016 Springer International Publishing Switzerland

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Murugan, S., Amaravadi, R.K. (2016). Methods for Studying Autophagy Within the Tumor Microenvironment. In: Koumenis, C., Coussens, L., Giaccia, A., Hammond, E. (eds) Tumor Microenvironment. Advances in Experimental Medicine and Biology, vol 899. Springer, Cham. https://doi.org/10.1007/978-3-319-26666-4_9

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