@article {MATSUBARA1427, author = {TAKUMA MATSUBARA and MARIKO URATA and TSUYOSHI NAKAJIMA and MARI FUKUZAKI and RYO MASUDA and YOSHIYUKI YOSHIMOTO and WILLIAM N. ADDISON and CHIHIRO NAKATOMI and KAZMASA MORIKAWA and MIN ZHANG and KATSURA SAEKI and YUKIKO TAKAHASHI and ATSUKO NAKAMICHI and SHOICHIRO KOKABU}, title = {Geranylgeraniol-induced Myogenic Differentiation of C2C12 Cells}, volume = {32}, number = {6}, pages = {1427--1431}, year = {2018}, doi = {10.21873/invivo.11395}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Geranylgeraniol (GGOH) is a C20 isoprenoid found in fruits, vegetables, and grains, including rice. As a food substance, GGOH is categorized as {\textquoteleft}Generally Recognized as Safe{\textquoteright}. GGOH is an intermediate product in the mevalonate pathway and acts as a precursor to geranylgeranyl pyrophosphate. Materials and Methods: C2C12 mouse myoblasts derived from muscle satellite cells were used. Quantitative reverse-transcriptase polymerase chain reaction, western blotting analysis, and immunocytochemical analysis were performed to respectively assess mRNA expression, protein levels, and the number of myofibers. Results: GGOH reduced the expression levels of skeletal muscle atrophy-related ubiquitin ligases in myofibers derived from C2C12 cells. GGOH induced myogenic differentiation of C2C12 cells via geranylgeranylation. GGOH did not adversely affect the proliferation of C2C12 cells. Conclusion: GGOH induces myoblast differentiation in C2C12 cells.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/32/6/1427}, eprint = {https://iv.iiarjournals.org/content/32/6/1427.full.pdf}, journal = {In Vivo} }