TY - JOUR T1 - Virome Analysis Reveals No Association of Head and Neck Vascular Anomalies with an Active Viral Infection JF - In Vivo JO - In Vivo SP - 1323 LP - 1331 DO - 10.21873/invivo.11382 VL - 32 IS - 6 AU - NORA FRANKE AU - MICHAEL BETTE AU - ANDRÉ MARQUARDT AU - THOMAS BRIESE AU - W. IAN LIPKIN AU - CHRISTOPHER KURZ AU - JOVINE EHRENREICH AU - ELISABETH MACK AU - BIANKA BAYING AU - VLADIMIR BENEŠ AU - FIONA R. RODEPETER AU - ANDREAS NEFF AU - AFSHIN TEYMOORTASH AU - BEHFAR EIVAZI AU - URBAN GEISTHOFF AU - BORIS A. STUCK AU - UDO BAKOWSKY AU - ROBERT MANDIC Y1 - 2018/11/01 UR - http://iv.iiarjournals.org/content/32/6/1323.abstract N2 - Background/Aim: Vascular anomalies encompass different vascular malformations [arteriovenous (AVM), lymphatic (LM), venous lymphatic (VLM), venous (VM)] and vascular tumors such as hemangiomas (HA). The pathogenesis of vascular anomalies is still poorly understood. Viral infection was speculated as a possible underlying cause. Materials and Methods: A total of 13 human vascular anomalies and three human skin control tissues were used for viral analysis. RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. The Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) was deployed on 10 tissues with vascular anomalies (2×AVM, 1×HA, 1×LM, 2×VLM, 4×VM). Results: RNA sequencing did not show any correlation of AVM with viral infection. By deploying VirCapSeq-VERT, no consistent viral association was seen in the tested tissues. Conclusion: The analysis does not point to the presence of an active viral infection in vascular anomalies. However, transient earlier viral infections, e.g. during pregnancy, cannot be excluded with this approach. ER -