PT - JOURNAL ARTICLE AU - NORA FRANKE AU - MICHAEL BETTE AU - ANDRÉ MARQUARDT AU - THOMAS BRIESE AU - W. IAN LIPKIN AU - CHRISTOPHER KURZ AU - JOVINE EHRENREICH AU - ELISABETH MACK AU - BIANKA BAYING AU - VLADIMIR BENEŠ AU - FIONA R. RODEPETER AU - ANDREAS NEFF AU - AFSHIN TEYMOORTASH AU - BEHFAR EIVAZI AU - URBAN GEISTHOFF AU - BORIS A. STUCK AU - UDO BAKOWSKY AU - ROBERT MANDIC TI - Virome Analysis Reveals No Association of Head and Neck Vascular Anomalies with an Active Viral Infection AID - 10.21873/invivo.11382 DP - 2018 Nov 01 TA - In Vivo PG - 1323--1331 VI - 32 IP - 6 4099 - http://iv.iiarjournals.org/content/32/6/1323.short 4100 - http://iv.iiarjournals.org/content/32/6/1323.full SO - In Vivo2018 Nov 01; 32 AB - Background/Aim: Vascular anomalies encompass different vascular malformations [arteriovenous (AVM), lymphatic (LM), venous lymphatic (VLM), venous (VM)] and vascular tumors such as hemangiomas (HA). The pathogenesis of vascular anomalies is still poorly understood. Viral infection was speculated as a possible underlying cause. Materials and Methods: A total of 13 human vascular anomalies and three human skin control tissues were used for viral analysis. RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. The Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) was deployed on 10 tissues with vascular anomalies (2×AVM, 1×HA, 1×LM, 2×VLM, 4×VM). Results: RNA sequencing did not show any correlation of AVM with viral infection. By deploying VirCapSeq-VERT, no consistent viral association was seen in the tested tissues. Conclusion: The analysis does not point to the presence of an active viral infection in vascular anomalies. However, transient earlier viral infections, e.g. during pregnancy, cannot be excluded with this approach.