TY - JOUR T1 - Podoplanin and PROX1 Expression in Hypercaloric Diet-induced Pancreatic Injuries JF - In Vivo JO - In Vivo SP - 1157 LP - 1163 DO - 10.21873/invivo.11586 VL - 33 IS - 4 AU - DELIA TARAN AU - VALERIA NICOLETA TARLUI AU - RALUCA AMALIA CEAUSU AU - ANCA MARIA CIMPEAN AU - MARIUS RAICA AU - SIMONA SARB Y1 - 2019/07/01 UR - http://iv.iiarjournals.org/content/33/4/1157.abstract N2 - Background: The role of podoplanin (PDPN) and homebox prospero gene 1 (PROX1) in early stages of pancreatic islet changes induced by hypercaloric diet is unclear. The aim of this study was to study PDPN and PROX1 variability in pancreatic islets after a hypercaloric diet in a rat experimental model. Materials and Methods: Pancreatic biopsies harvested from Sprague–Dawley rats at 3, 6, and 9 weeks following hypercaloric diet intake were evaluated for morphological and molecular changes of Langerhans islets based on PDPN and PROX1 expression Results: Six weeks of hypercaloric diet induced hypertrophy of pancreatic islets with focal expression of Pdpn and Prox1 mRNA. At 9 weeks of hypercaloric diet, strong peri-insular inflammation was found around hypertrophic islets highly expressing PDPN, and lacking Prox1 mRNA and protein expression. Conclusion: This is the first report of Pdpn and Prox1 mRNA expression variability and involvement in early steps of pancreatic islet changes following hypercaloric food intake. ER -