TY - JOUR T1 - Development of a Modified Surgical Technique for Simulating Ischemic Cerebral Cortex Injury in Rats JF - In Vivo JO - In Vivo SP - 1175 LP - 1181 DO - 10.21873/invivo.11588 VL - 33 IS - 4 AU - CHIEN-FU YEH AU - TUNG-YUEH CHUANG AU - YU-WEN HUNG AU - MING-YING LAN AU - CHING-HAN TSAI AU - HAO-XIANG HUANG AU - YUNG-YANG LIN Y1 - 2019/07/01 UR - http://iv.iiarjournals.org/content/33/4/1175.abstract N2 - Background/Aim: Middle cerebral artery occlusion (MCAO) in rodents is an essential animal model for research focusing on ischemic stroke. To date, several kinds of surgical methods for MCAO have been developed and the craniotomy method has the advantage of direct visualization of the middle cerebral artery (MCA). MCAO at a more proximal site produces better surgical results, but it is a more invasive technique. The aim of this study was to evolve the surgical technique for simulating ischemic cerebral cortex injury in rats. Materials and Methods: To approach proximal MCA with a less invasive procedure, a modified surgical technique for MCAO in rats was developed. Besides, rats receiving the modified and conventional method were compared with regard to infarct volume and by behavioral tests. Results: Following craniotomy, we proposed that the inferior edge of the craniotomy should be enlarged with fine forceps. This modified surgical method induces larger infarct volume, significant behavioral impairment and can induce ischemic stroke. Additionally, it does not significantly increase the operation time, and has produced no obvious complications. Conclusion: This modified surgical technique may serve as a practical method for performing MCAO. ER -