TY - JOUR T1 - Resveratrol Attenuates Nicotine-mediated Oxidative Injury by Inducing Manganese Superoxide Dismutase in Renal Proximal Tubule Cells JF - In Vivo JO - In Vivo SP - 551 LP - 555 VL - 31 IS - 4 AU - SAMUEL HALL AU - MEHUL DIXIT AU - ISTVAN ARANY Y1 - 2017/07/01 UR - http://iv.iiarjournals.org/content/31/4/551.abstract N2 - Background/Aim: Nicotine (NIC) exposure – via smoking and the increasingly popular E-cigarettes- increases oxidative stress and hence, renal risk in smokers. Resveratrol (RES) may help ameliorate this risk by mounting anti-oxidant responses in the kidney. Materials and Methods: Renal proximal tubule cells (NRK52E) were treated with vehicle or 20 μM RES prior to treatment with 200 μM NIC and generation of reactive oxygen species (ROS) as well as cell viability was determined. RES-induced antioxidant responses were determined in reporter luciferase assays. Gene silencing was used to determine mechanism of RES action. Results: RES protected NRK52E cells from NIC-induced oxidative injury. RES activated the promoter of the anti-oxidant manganese superoxide dismutase (MnSOD) gene via activation of the forkhead box O (FoxO3a) transcription factor. Silencing of MnSOD abolished the protective effects of RES on NIC-associated oxidative injury. Conclusion: RES may provide protection to the kidney from the adverse effects of NIC in smokers. ER -