PT  - JOURNAL ARTICLE
AU  - KANG, KYUNG-KU
AU  - LEE, EUN-JOO
AU  - KIM, YOUG-DEUK
AU  - CHUNG, MYUNG-JIN
AU  - KIM, JUN-YOUNG
AU  - KIM, SHIN-YOON
AU  - HWANG, SU-KYEUNG
AU  - JEONG, KYU-SHIK
TI  - Vitamin C Improves Therapeutic Effects of Adipose-derived Stem Cell Transplantation in Mouse Tendonitis Model
DP  - 2017 May 01
TA  - In Vivo
PG  - 343--348
VI  - 31
IP  - 3
4099  - http://iv.iiarjournals.org/content/31/3/343.short
4100  - http://iv.iiarjournals.org/content/31/3/343.full
SO  - In Vivo2017 May 01; 31
AB  - Background/Aim: We hypothesized that combined therapy using adipose-derived stem cells (ASCs) and vitamin C might improve tendon regeneration in tendonitis. To determine combined effects of ASC transplantation with vitamin C, we used senescence marker protein 30 (SMP30)-knockout (KO) mice that cannot biosynthesize vitamin C by themselves. Materials and Methods: SMP30-KO were divided into four groups: Control, vitamin C, ASCs, and vitamin C plus ASCs. Tendonitis was induced in the achilles tendons via injection of collagenase type I. After 1 week, ASCs were injected into the intratendonal region. After 30 days, all mice were sacrificed and Achilles tendons were isolated. Results: Gross and microscopic findings showed mice treated with combination of ASC transplantation and vitamin C showed better tendon regeneration than those in other groups. This combination led to higher serum vitamin C levels than use of vitamin C alone. This indicates that the vitamin C-treated group used more vitamin C as a precursor to collagen synthesis, whereas vitamin C was in excess in the combination group because of the added effect of ASCs on tendon healing. Conclusion: This study showed that vitamin C improved the effect of ASC transplantation on tendonitis by inducing a better stem cell niche.