TY - JOUR T1 - Automated Multichamber Time-lapse Videography for Long-term <em>In Vivo</em> Observation of Migrating Cells JF - In Vivo JO - In Vivo SP - 329 LP - 334 VL - 31 IS - 3 AU - HELMUT BUHLER AU - RAPHAEL ADAMIETZ AU - THERESA ABELN AU - DAVID DIAZ-CARBALLO AU - PASCALINE NGUEMGO-KOUAM AU - THOMAS HERO AU - IRENAUS A. ADAMIETZ Y1 - 2017/05/01 UR - http://iv.iiarjournals.org/content/31/3/329.abstract N2 - Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. Materials and Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with &lt;1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. Results: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. Conclusion: This study demonstrates that useful videographic systems can be constructed at low cost. ER -