RT Journal Article SR Electronic T1 Automated Multichamber Time-lapse Videography for Long-term In Vivo Observation of Migrating Cells JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 329 OP 334 VO 31 IS 3 A1 HELMUT BUHLER A1 RAPHAEL ADAMIETZ A1 THERESA ABELN A1 DAVID DIAZ-CARBALLO A1 PASCALINE NGUEMGO-KOUAM A1 THOMAS HERO A1 IRENAUS A. ADAMIETZ YR 2017 UL http://iv.iiarjournals.org/content/31/3/329.abstract AB Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. Materials and Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with <1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. Results: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. Conclusion: This study demonstrates that useful videographic systems can be constructed at low cost.