<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BUHLER, HELMUT</style></author><author><style face="normal" font="default" size="100%">ADAMIETZ, RAPHAEL</style></author><author><style face="normal" font="default" size="100%">ABELN, THERESA</style></author><author><style face="normal" font="default" size="100%">DIAZ-CARBALLO, DAVID</style></author><author><style face="normal" font="default" size="100%">NGUEMGO-KOUAM, PASCALINE</style></author><author><style face="normal" font="default" size="100%">HERO, THOMAS</style></author><author><style face="normal" font="default" size="100%">ADAMIETZ, IRENAUS A.</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Automated Multichamber Time-lapse Videography for Long-term &lt;em&gt;In Vivo&lt;/em&gt; Observation of Migrating Cells</style></title><secondary-title><style face="normal" font="default" size="100%">In Vivo</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2017-05-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">329-334</style></pages><volume><style face="normal" font="default" size="100%">31</style></volume><issue><style face="normal" font="default" size="100%">3</style></issue><abstract><style  face="normal" font="default" size="100%">Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. Materials and Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with &lt;1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. Results: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. Conclusion: This study demonstrates that useful videographic systems can be constructed at low cost.</style></abstract></record></records></xml>