RT Journal Article SR Electronic T1 Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 279 OP 285 VO 32 IS 2 A1 TSUNG-HSIEN YEN A1 CHIA-LING HSIEH A1 TSU-TE LIU A1 CHIH-SHENG HUANG A1 YEN-CHUNG CHEN A1 YAO-CHEN CHUANG A1 SONG-SHEI LIN A1 FEI-TING HSU YR 2018 UL http://iv.iiarjournals.org/content/32/2/279.abstract AB The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.